A novel virus isolated from the aphid Brevicoryne brassicae with similarity to Hymenoptera picorna-like viruses
A novel virus isolated from the aphid Brevicoryne brassicae with similarity to Hymenoptera picorna-like viruses
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DOI：
10.1099/vir.0.83050-0
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发表时间：
2007-09-01
影响因子：
3.8
通讯作者：
Ryabov, Eugene V.
中科院分区：
文献类型：
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作者：
Ryabov, Eugene V.
利用随机扩增衣壳化RNA的方法，在白菜蚜虫中鉴定出一种新的病毒BrBV。BrBV病毒RNA基因组的完整序列已经确定。正链基因组RNA长度为10 161 nt（不包括3' poly(A)尾巴），包含一个开放阅读框（位置793-9744），编码一个推测为2983 aa的多蛋白。该多蛋白的n端部分与黄病毒的结构蛋白相似。c端部分具有与黄病毒和其他小核糖核酸样病毒相似的解旋酶、半胱氨酸蛋白酶和RNA依赖的RNA聚合酶的一致序列。观察到的序列相似性最高的是来自蜜蜂和一种内寄生黄蜂的黄酮类病毒。BrBV的复制和传播不依赖于蚜虫的寄生蜂侵染。
A novel virus, Brevicoryne brassicae virus (BrBV), has been identified in the cabbage aphid using a method based on the random amplification of encapsidated RNA. The complete sequence of the RNA genome of BrBV has been determined. The positive-strand genomic RNA is 10 161 nt, excluding the 3' poly(A) tail, and contains a single open reading frame (positions 793-9744) encoding a putative polyprotein of 2983 aa. The N-terminal part of the polyprotein shows similarity with the structural proteins of iflaviruses. The C-terminal part possesses consensus sequences of the helicase, cysteine protease and RNA-dependent RNA polymerase similar to those of iflaviruses and other picorna-like viruses. The highest sequence similarity observed was with iflaviruses from honeybee and an endoparasitic wasp. Replication and transmission of BrBV was not dependent on endoparasitic wasp infestation of the aphids.