INSITU RECOVERY OF BUTANOL DURING FERMENTATION .2. FED-BATCH EXTRACTIVE FERMENTATION
INSITU RECOVERY OF BUTANOL DURING FERMENTATION .2. FED-BATCH EXTRACTIVE FERMENTATION
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DOI：
10.1007/bf00387326
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
WILKE, CR
中科院分区：
文献类型：
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作者：
ROFFLER, SR;BLANCH, HW;WILKE, CR
通过萃取发酵连续去除发酵液中的丙酮和丁醇，降低了丙酮丁醇发酵中的终产物抑制。从丙酮丁醇梭菌中原位去除抑制产物导致反应器生产率增加;丁醇体积生产率从分批发酵中的0.58 kg/（m3 h）增加到使用油醇作为提取溶剂的补料分批提取发酵中的1.5 kg/（m3 h）。使用分批补料操作允许发酵高达500 kg/m3的葡萄糖溶液，导致废水量减少3.5至5倍。在发酵结束时，丁醇在油醇提取剂中达到30-35 kg/m3的浓度，该浓度比常规分批或补料分批发酵中可能的浓度高2-3倍。丁醇生产率和葡萄糖转化率在补料分批提取发酵中与连续发酵和原位产物去除发酵相比是有利的。
End-product inhibition in the acetone-butanol fermentation was reduced by using extractive fermentation to continuously remove acetone and butanol from the fermentation broth. In situ removal of inhibitory products from Clostridium acetobutylicum resulted in increased reactor productivity; volumetric butanol productivity increased from 0.58 kg/(m3h) in batch fermentation to 1.5 kg/(m3h) in fed-batch extractive fermentation using oleyl alcohol as the extraction solvent. The use of fed-batch operation allowed glucose solutions of up to 500 kg/m3to be fermented, resulting in a 3.5- to 5-fold decrease in waste water volume. Butanol reached a concentration of 30–35 kg/m3in the oleyl alcohol extractant at the end of fermentation, a concentration that is 2–3 times higher than is possible in regular batch or fed-batch fermentation. Butanol productivities and glucose conversions in fed-batch extractive fermentation compare favorable with continuous fermentation and in situ product removal fermentations.