Maximization of skin capillaries during intravital video-microscopy in essential hypertension: comparison between venous congestion, reactive hyperaemia and core heat load tests
Maximization of skin capillaries during intravital video-microscopy in essential hypertension: comparison between venous congestion, reactive hyperaemia and core heat load tests
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DOI：
10.1042/cs19990097
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发表时间：
1999-10-01
期刊：
影响因子：
6
通讯作者：
MacGregor, GA
中科院分区：
文献类型：
--
作者：
Antonios, TFT;Rattray, FEM;MacGregor, GA
活体毛细血管视频显微镜是研究皮肤毛细血管的动态方法。直接活体显微镜技术（无染料）取决于毛细血管内红细胞的存在，以进行识别。本研究的目的是比较不同的技术，试图建立最好的方法，最大限度地增加可见灌注毛细血管的数量在活体毛细血管显微镜。我们比较了静脉充血与闭塞后反应性充血的影响（研究1）。我们还研究了静脉充血，然后首先进行闭塞后反应性充血，然后进行核心热负荷试验（研究2）。最后，我们研究了静脉充血，随后是闭塞后反应性充血和静脉充血（研究3）。在研究1中，毛细血管密度随着静脉充血从基线值74 +/- 2（平均值+/- S.E.M.）每视野82 +/- 3个（P < 0.0001;方差分析）。反应性充血时，可见毛细血管密度明显降低至69+/-2/视野。在研究2中，基线毛细血管密度为69+/-4/视野，随着静脉充血显著增加至74 +/- 4/视野（P = 0.01）。在反应性充血和核心热负荷的情况下，表观密度为62 +/- 4/视野。在研究3中，基线密度为70 +/- 2/视野，随着静脉充血显著增加至80 +/- 3/视野（P < 0.0001）。反应性充血合并静脉充血时，密度为81 +/- 3/视野（与单独静脉充血相比，P = 0.328）。结果表明，静脉充血在60毫米汞柱2分钟是最有效的方法，可视化的最大数量的灌注皮肤毛细血管在活体视频显微镜。
Intravital capillary video-microscopy is a dynamic method for studying skin capillaries. The technique of direct intravital microscopy (without dyes) depends on the presence of red blood cells inside capillaries for their identification. The aim of the present study was to compare different techniques to try to establish the best method for maximizing the number of visible perfused capillaries during intravital capillary microscopy. We compared the effects of venous congestion with those of post-occlusive reactive hyperaemia (Study 1). We also investigated venous congestion followed fi rst by post-occlusive reactive hyperaemia and then by a core heat load test (Study 2). Finally we investigated venous congestion followed by post-occlusive reactive hyperaemia combined with venous congestion (Study 3). In Study 1, capillary density increased with venous congestion from a baseline value of 74 +/- 2 (mean +/- S.E.M.) per field to 82 +/- 3 per field (P < 0.0001; analysis of variance). With reactive hyperaemia, there was an apparent decrease in visible capillary density to 69+/-2 per field. In Study 2, baseline capillary density was 69+/-4 per field, and this increased significantly with venous congestion to 74 +/- 4 per field (P = 0.01). With both reactive hyperaemia and core heat load, the apparent density was 62 +/- 4 per field. In Study 3 the baseline density was 70 +/- 2 per field, and this increased significantly with venous congestion to 80 +/- 3 per field (P < 0.0001). With reactive hyperaemia combined with venous congestion, the density was 81 +/- 3 per field (P = 0.328 compared with venous congestion alone). The results show that venous congestion at 60 mmHg for 2 min is the most effective method for visualization of the maximal number of perfused skin capillaries during intravital video-microscopy.