O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner
O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner
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DOI：
10.1046/j.1432-1033.2003.03571.x
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发表时间：
2003-05-01
期刊：
影响因子：
--
通讯作者：
Sakano, H
中科院分区：
文献类型：
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作者：
Oka, Y;Kobayakawa, K;Sakano, H
在啮齿类动物中，嗅觉上皮（OE）可以分为四个地形不同的区域，并且气味受体（OR）基因家族的每个成员仅在一个特定区域表达。为了研究OE区域结构的功能意义，我们使用差异显示方法寻找区域特异性表达的基因。在从大鼠OE中分离的克隆中，我们鉴定了一种名为O-MACS的新型嗅觉蛋白，它是中链酰基辅酶a合成酶家族的成员。o-macs基因编码580个氨基酸的蛋白，与MACS家族其他蛋白共享56-63%的同源性。RT-PCR分析表明，o-macs基因与其他MACS家族基因不同，仅在OE中表达。原位杂交发现o-macs转录本存在于嗅觉感觉神经元（OSNs）的神经元细胞层以及OE最背内侧区域（1区）的支持细胞层和基底细胞层中。发育分析显示，o-macs基因在胚胎第11.5天就已经表达，在OR基因表达开始之前，在大鼠嗅觉基因的一个限制区域内表达。带有c-Myc和His6标记的重组O-MACS蛋白具有酰基辅酶a合成酶活性，可激活脂肪酸，并像其他MACS家族蛋白一样定位于线粒体。目前的研究表明，这种新蛋白可能在以特定区域的方式处理气味或在发育过程中OE的区域模式中发挥重要作用。
In rodents, the olfactory epithelium (OE) can be divided into four topographically distinct zones, and each member of the odorant receptor (OR ) gene family is expressed only in one particular zone. To study the functional significance of the zonal structure of the OE, we searched for genes expressed in a zone-specific manner by using the differential display method. Among the clones isolated from the rat OE, we characterized a novel olfactory protein termed O-MACS, a member of the medium-chain acyl-CoA synthetase family. The o-macs gene encodes a protein of 580 amino acids, sharing 56-63% identity with other MACS family proteins. RT-PCR analysis demonstrated that the o-macs gene is expressed only in the OE, unlike other MACS family genes. In situ hybridization revealed that the o-macs transcripts are present in the neuronal cell layer of olfactory sensory neurons (OSNs) as well as in the supporting and basal cell layers in the most dorso-medial area (zone 1) of the OE. Developmental analysis revealed that the o-macs gene is already expressed on embryonic day 11.5, before the onset of the OR gene expression, in a restricted area within the rat olfactory placode. Recombinant O-MACS protein tagged with c-Myc and His6 demonstrated an acyl-CoA synthetase activity for fatty acid activation, and protein localization to mitochondria like other MACS family proteins. The present study indicates that this novel protein may play important roles in processing odorants in a zone-specific manner, or the zonal patterning of the OE during development.