Single‐strand breaks, chromosome aberrations, sister‐chromatid exchanges, and micronuclei in blood lymphocytes of workers exposed to styrene during the production of reinforced plastics
Single‐strand breaks, chromosome aberrations, sister‐chromatid exchanges, and micronuclei in blood lymphocytes of workers exposed to styrene during the production of reinforced plastics
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强化塑料生产过程中接触苯乙烯的工人血液淋巴细胞中的单链断裂、染色体畸变、姐妹染色单体交换和微核
DOI：
10.1002/em.2850170105
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发表时间：
1991
影响因子：
2.8
通讯作者：
F. Oleson
中科院分区：
文献类型：
--
作者：
J. Mäki‐Paakkanen;H. Norppa;Walles Sa;S. Osterman;F. Oleson
分析了17名工人和17名对照受试者血液淋巴细胞的染色体畸变（CA）、微核（MN，胞质分裂阻滞[CB]法）和姐妹染色单体交换（SCE）。尿中扁桃酸平均浓度（9.4 mmol/l）和血液中苯乙二醇平均浓度（2.5 μmol/l）表明，该工厂中苯乙烯的暴露量约为300 mg/m3。与不吸烟的对照组相比，不吸烟的工人中CA的数量显著较高。所有工人的接触时间和个体CA水平之间存在显著相关性。在MN或SCE中未观察到显著影响。通过DNA解旋技术，在9名工人和8名对照中研究了分离的淋巴细胞DNA中的单链断裂（SSB）。结果表明，SSB在接触工人中增加。目前的研究结果支持早期报告的增强塑料行业的工人的血液淋巴细胞中的结构CA的增加，也表明，SSB升高，在这样的工人。
Chromosome aberrations (CA), micronuclei (MN, cytokinesis‐block [CB] method), and sister‐chromatid exchanges (SCE) were analysed in blood lymphocytes of 17 workers and 17 control subjects. The mean urinary mandelic acid level (average 9.4 mmol/1) and styrene glycol in blood (average 2.5 μmol/1) implied exposure to about 300 mg/m3 of styrene in the plant. The number of CA was significantly higher in non‐smoking workers compared with nonsmoking controls. A significant correlation was observed between duration of exposure and individual CA level of all workers. No significant effects were observed in MN or SCE. Single‐strand breaks (SSB) in DNA of isolated lymphocytes were studied in nine of the workers and eight of the controls by the DNA‐unwinding technique. The results showed an increase in SSB among the exposed workers. The present findings support earlier reports on the increase of structural CA in blood lymphocytes of workers in the reinforced plastic industry, and also show that SSBs are elevated in such workers.