Effect of tetrachlorodibenzo-p-dioxin (TCDD) on the glucuronidation of retinoic acid in the rat.
Effect of tetrachlorodibenzo-p-dioxin (TCDD) on the glucuronidation of retinoic acid in the rat.
复制标题
四氯二苯并-对-二恶英（TCDD）对大鼠视黄酸葡萄糖醛酸化的影响。
DOI：
10.1016/0304-4165(89)90134-7
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Zile,MH
中科院分区：
文献类型：
--
作者：
Bank,PA;Salyers,KL;Zile,MH
雄性大鼠单次口服四氯二苯并对二英（TCDD）10 μg/kg后，肝脏中的视黄酯减少了33%，但肾脏中的视黄酯增加了13倍，血清中的视黄醇增加了3倍。肝和肾微粒体尿苷二磷酸葡萄糖醛酸转移酶（UDPGT）对全反式视黄酸的活性增加3.7倍和2.6倍，分别为10天后，接触TCDD。通过与真实RG在反相高压液相色谱（HPLC）上共色谱法验证[3 H]视黄酰基β-葡萄糖醛酸苷（RG）的体外形成，鉴别视黄酸为β-葡萄糖醛酸酶处理后的水解产物，并通过负碎片质谱法、快速原子结合表征全反式视黄酰基葡萄糖醛酸苷。我们的结论是，增加视黄酸葡萄糖醛酸化可能是一个促成因素，肝脏消耗的维生素A和增加排泄的维生素A代谢产物后，TCDD曝光。
Administration of a single oral dose (10 μg/kg) of tetrachlorodibenzo-p-dioxin (TCDD) caused a 33% decrease in retinyl esters in the livers of male rats, but a 13-fold increase in retinyl esters in the kidney and a 3-fold increase in serum retinol. Liver and kidney microsomal uridine diphosphoglucuronosyltransferase (UDPGT) activity toward all-trans-retinoic acid was increased 3.7- and 2.6-fold, respectively, ten days following exposure to TCDD. Verification of the in vitro formation of [3H]retinoyl β-glucuronide (RG) was by cochromatography with authenic RG on reversed phase high pressure liquid chromatography (HPLC), identification of retinoic acid as the hydrolysis product after β-glucuronidase treatment, and the characterization of the all-trans-retinoyl glucuronide by negative fragment mass spectroscopy, fast atom bobardment. We conclude that increased retinoic acid glucuronidation may be a contributing factor to the hepatic depletion of vitamin A and the increased excretion of vitamin A metabolites following TCDD exposure.