Depression of iron uptake from transferrin by isolated hepatocytes in the presence of ethanol is a pH-dependent consequence of ethanol metabolism.
Depression of iron uptake from transferrin by isolated hepatocytes in the presence of ethanol is a pH-dependent consequence of ethanol metabolism.
复制标题
在乙醇存在下，分离的肝细胞对转铁蛋白的铁吸收抑制是乙醇代谢的 pH 依赖性结果。
DOI：
10.1111/j.1530-0277.1986.tb05125.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Potter,BJ
中科院分区：
文献类型：
--
作者：
Beloqui,O;Nunes,RM;Blades,B;Berk,PD;Potter,BJ
在含有 10 mM 乙醇的弱缓冲培养基中，将新鲜分离的大鼠肝细胞与高度纯化的放射性标记的大鼠转铁蛋白一起孵育，导致这些细胞对铁的吸收明显减少，与无乙醇对照研究相比，pH 值下降幅度更大。即使在添加转铁蛋白之前将细胞与乙醇预孵育 90 分钟，这种对铁吸收的影响仍然存在。增加系统的缓冲能力或添加乙醇脱氢酶代谢抑制剂（4-甲基吡唑）可使铁吸收恢复到对照值。乙醛、乙酸盐、乳酸盐（乙醇代谢产物）和3-丁醇（不被乙醇脱氢酶代谢的酒精）对铁的吸收没有影响。对 pH 范围 6-8.5 内铁吸收的进一步研究表明，铁吸收对细胞外 pH 值有明显依赖性。亮氨酸掺入细胞蛋白也被发现具有 pH 依赖性，这表明，根据目前对其他细胞类型对转铁蛋白回收的理解，在酗酒者中观察到的铁稳态紊乱可以部分归因于其酸碱代谢的改变。
Incubation of freshly isolated rat hepatocytes with highly purified radiolabeled rat transferrin in weakly buffered medium in the presence of 10 mM ethanol resulted in a marked diminution of iron uptake by these cells, associated with a greater pH depression than in ethanol‐free control studies. This effect on iron uptake persisted, even when the cells were preincubated for 90 min with ethanol before the addition of transferrin. Increasing the buffering capacity of the system or the addition of a metabolic inhibitor of alcohol dehydrogenase (4‐methylpyrazole) returned iron uptake to control values. Acetaktehyde, acetate, lactate (products of ethanol metabolism), and 3‐butanol (an alcohol not metabolized by alcohol dehydrogenase) had no influence on iron uptake. Further investigation of iron uptake over the pH range 6–8.5 revealed a marked dependency of iron uptake on the extracellular pH. Leucine incorporation into cell protein was also found to be pH dependent ft is suggested that, in the light of current understanding of transferrin recycling by other cell types, the disturbances of iron homeostasis observed in alcoholics can be partially accounted for by alterations in their acid‐base metabolism.