Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity, and line tension.
Shape analysis of giant vesicles with fluid phase coexistence by laser scanning microscopy to determine curvature, bending elasticity, and line tension.
复制标题
通过激光扫描显微镜对液相共存的巨型囊泡进行形状分析，以确定曲率、弯曲弹性和线张力。
DOI：
10.1007/978-1-59745-519-0_25
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Baumgart,Tobias
中科院分区：
文献类型：
--
作者：
Hess,SamuelT;Gudheti,ManasaV;Mlodzianoski,Michael;Baumgart,Tobias
Membrane shape parameters such as curvature, bending elasticity, and lateral tension, are relevant to the lateral organization and function of biomembranes, and may critically influence the formation of lateral clustering patterns observed in living cells. Fluorescence laser-scanning microscopy can be used to image vesicles and cell membranes, and from shape analysis of these images mechanical membrane parameters can be quantified. Methods to analyze images of equatorial sections obtained by confocal or multiphoton microscopy are detailed, in order to estimate curvature, lateral tension, line tension, relative differences in mean curvature and Gaussian curvature bending moduli, and fluorescence dye intensity profiles, typically within coexisting liquid-ordered and liquid-disordered membrane domains. A variety of shape tracing and shape fitting methods are compared.
