ACTION OF INSECT HORMONES AT CELLULAR LEVEL - MORPHOLOGICAL CHANGES OF A DIPLOID CELL LINE OF DROSOPHILA-MELANOGASTER, TREATED WITH ECDYSONE AND SEVERAL ANALOGS IN-VITRO
ACTION OF INSECT HORMONES AT CELLULAR LEVEL - MORPHOLOGICAL CHANGES OF A DIPLOID CELL LINE OF DROSOPHILA-MELANOGASTER, TREATED WITH ECDYSONE AND SEVERAL ANALOGS IN-VITRO
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DOI：
10.1016/0014-4827(72)90384-9
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发表时间：
1972-01-01
影响因子：
3.7
通讯作者：
COURGEON, AM
中科院分区：
文献类型：
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作者：
COURGEON, AM
用黑腹果蝇胚胎培养的二倍体细胞在实验室中保存3年以上，对人工合成的蜕皮激素α-蜕皮激素和β-蜕皮激素以及几种蜕皮激素类似物有特异性反应。细胞对β-蜕皮激素最敏感，对低至0.006 μg/ml的剂量有反应。β-蜕皮酮的活性是肌甾酮和半甾酮的5 ~ 10倍，α-蜕皮酮的活性是α-蜕皮酮的500倍，22-异蜕皮酮的5000倍。激素处理的细胞表现出一系列形态变化，这些变化与所有测试的蜕皮激素类似物相同：在引入激素16至24小时后，细胞可以看到变平或呈纺锤形。然后它们倾向于聚集成纤维或变得非常扁平，膜显示出明显的扩张。细胞随后出现充满各种包涵体。这种进化的速度取决于剂量。这种形态进化需要在培养基中存在胎牛血清，并且当细胞达到成纤维细胞阶段时变得不可逆，这与增殖的停止相一致。抗蜕皮激素细胞系已被分离出来。
Cells of a diploid line established from embryos ofDrosophila melanogaster, and maintained in the laboratory for more than 3 years, respond specifically to the synthetic moulting hormones α-ecdysone and β-ecdysone and to several ecdysone analogues. The cells are most sensitive to β-ecdysone and respond to doses as low as 0.006 μg/ml. β-Ecdysone was found to be 5 or 10 times more active than inokosterone and cyasterone, 500 times more active than α-ecdysone, and 5 000 times more than 22-iso-ecdysone.Hormone-treated cells display a series of morphological modifications which are identical for all the ecdysone analogues tested: 16 to 24 h after introduction of the hormone, the cells are seen to flatten or take a spindle shape. Then they tend to aggregate and become fibroblastic or very flattened, and the membrane shows an apparent expansion. The cells appear afterwards full of various inclusions. The rapidity of such an evolution is dose dependent.This morphological evolution requires the presence of foetal calf serum in the culture medium, and becomes irreversible when the cells have attained the fibroblastic stage, which coincides with an arrest of multiplication.Lines of ecdysone-resistant cells have been isolated.