Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation.
Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation.
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通过冷冻替代固定更好地保存培养的巨噬细胞和吞噬的酵母细胞的精细结构的便捷方法。
DOI：
10.1093/jmicro/dfx006
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Chibana H.
中科院分区：
文献类型：
--
作者：
Yamaguchi M;Takahashi-Nakaguchi A;Aida Y;Sato-Okamoto M;Chibana H.
戊二醛固定后的快速冷冻-冷冻置换法（CF-FS法）获得了巨噬细胞和吞噬酵母细胞的自然和精细结构。在置于培养皿中的单孔钼网格上培养巨噬细胞使得通过“开放三明治法”快速冷冻细胞成为可能。当快速冷冻不能立即进行时，或者当实验室中没有快速冷冻装置时，这种方法可能很方便。
Rapid freeze–freeze substitution after glutaraldehyde fixation (CF–FS method) obtained the natural and fine structures of macrophages and engulfed yeast cells. Culturing macrophages on single hole molybdenum grids placed in culture dishes made possible the rapid freezing of cells by the ‘open sandwich method’. This method may be convenient when rapid-freezing cannot be performed immediately, or when a rapid-freezing device is not available in the lab.