ESTABLISHMENT AND REFINEMENT OF SEGMENTAL PATTERN IN THE DROSOPHILA EMBRYO - SPATIAL CONTROL OF ENGRAILED EXPRESSION BY PAIR-RULE GENES
ESTABLISHMENT AND REFINEMENT OF SEGMENTAL PATTERN IN THE DROSOPHILA EMBRYO - SPATIAL CONTROL OF ENGRAILED EXPRESSION BY PAIR-RULE GENES
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DOI：
10.1101/gad.1.10.1212
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发表时间：
1987-12-01
影响因子：
10.5
通讯作者：
OFARRELL, PH
中科院分区：
文献类型：
--
作者：
DINARDO, S;OFARRELL, PH
我们正在通过engrailed的免疫定位研究果蝇节段体模式的发展，engrailed是一种维持胚胎节段细分的发育调节蛋白，在机体模式建立和完善过程中以空间受限（条纹）的方式持续表达。对规则分割基因之间的调控网络建立了条带化表达模式。一般来说，特定成对规则基因的突变会影响偶数或奇数纠缠条纹。例如，fushi tarazu或奇对突变删除偶数条纹，而成对突变删除奇数条纹。对其他突变体（包括偶跳过奇跳过双突变体）的嵌入表达的分析表明，一些配对规则基因在确定嵌入条纹的正确宽度和位置方面发挥了作用。综上所述，嵌合模式的改变对最终的胚胎体模式有影响。因此，配对规则基因座通过遗传作用，建立了身体模式的早期总体轮廓。然而，在一些成对规则突变体中，纠缠模式是动态的，这表明，随着后来的事件建立在这个一般规则之上，形成最终的身体模式，对早期的成对规则缺陷进行调整——也就是说，模式进行调节。
We are examining the development of the segmental body pattern of Drosophila by immunolocalization of engrailed, a developmental regulatory protein that maintains segmental subdivisions in the embryo, and is expressed in a spatially restricted (striped) manner that persists while the body pattern is being established and refined. A regulatory network among pair-rule segmentation genes establishes the striped pattern of engrailed expression. In general, mutations in particular pair-rule genes affect either even- or odd-numbered engrailed stripes. For example, fushi tarazu or odd-paired mutations delete even-numbered stripes, whereas paired mutations delete odd-numbered stripes. An analysis of engrailed expression in other mutants, including even-skipped odd-skipped double mutants, indicates that some pair-rule genes play a rule in establishing the correct width and position of engrailed stripes. Overall, the changes in engrailed pattern have consequences for final embryonic body pattern. Thus, the pair-rule loci, acting through engrailed, establish an early, general outline of body pattern. However, in several pair-rule mutants, engrailed patterns are dynamic, suggesting that as later events build upon this general rule to form the final body pattern, adjustments are made in response to the earlier pair-rule defect--that is, the pattern regulates.