Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages.
Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages.
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DOI：
10.1038/srep22020
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发表时间：
2016-02-25
影响因子：
4.6
通讯作者：
Applegate LA
中科院分区：
文献类型：
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作者：
Abdel-Sayed P;Kaeppeli A;Siriwardena T;Darbre T;Perron K;Jafari P;Reymond JL;Pioletti DP;Applegate LA
多重耐药的铜绿假单胞菌已经逐渐增加，并阻碍了烧伤患者死亡率的进一步下降。这类伤口感染是医院感染的细菌储存库，并与显著的发病率和成本相关。抗微生物多阳离子树状大分子G3KL和G3RL能够杀死多重耐药的铜绿假单胞菌，以前已经开发过。这些树枝状大分子与一类由祖细胞皮肤细胞制成的生物绷带相结合，可以分泌生长因子，可以对伤口愈合过程产生积极影响。然而，聚阳离子已被用作抑制肿瘤的抗血管生成剂。由于新生血管在深度烧伤创面愈合过程中起着关键作用，因此使用抗微生物树枝状大分子可能会阻碍愈合过程。令人惊讶的是，我们在这项研究中发现，G3KL和G3RL树枝状大分子可以具有血管生成作用。此外，我们还表明，树枝状大分子浓度在50到100 μg/mL之间与生物绷带结合可以在不改变细胞活力的情况下抑制细菌生长长达5天。这些结果表明，抗菌树枝状大分子可以与生物绷带结合使用，并有可能通过增强血管生成来改善愈合过程。
Multi-drug resistant Pseudomonas aeruginosa has increased progressively and impedes further regression in mortality in burn patients. Such wound infections serve as bacterial reservoir for nosocomial infections and are associated with significant morbidity and costs. Anti-microbial polycationic dendrimers G3KL and G3RL, able to kill multi-drug resistant P. aeruginosa, have been previously developed. The combination of these dendrimers with a class of biological bandages made of progenitor skin cells, which secrete growth factors, could positively impact wound-healing processes. However, polycations are known to be used as anti-angiogenic agents for tumor suppression. Since, neovascularization is pivotal in the healing of deep burn-wounds, the use of anti-microbial dendrimers may thus hinder the healing processes. Surprisingly, we have seen in this study that G3KL and G3RL dendrimers can have angiogenic effects. Moreover, we have shown that a dendrimer concentration ranging between 50 and 100 μg/mL in combination with the biological bandages can suppress bacterial growth without altering cell viability up to 5 days. These results show that antimicrobial dendrimers can be used in combination with biological bandages and could potentially improve the healing process with an enhanced angiogenesis.