The influence of Gelatin/PCL ratio and 3-D construct shape of electrospun membranes on cartilage regeneration
The influence of Gelatin/PCL ratio and 3-D construct shape of electrospun membranes on cartilage regeneration
复制标题
明胶/PCL 比例和电纺膜 3-D 结构形状对软骨再生的影响
DOI：
10.1016/j.biomaterials.2013.09.082
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发表时间：
2014-01-01
期刊：
影响因子：
14
通讯作者：
Zhou, Guangdong
中科院分区：
文献类型：
--
作者：
Zheng, Rui;Duan, Huichuan;Zhou, Guangdong
支架在引导三维软骨再生中起着重要的作用。我们最近的研究报告了电纺明胶/聚己内酯(GT/PCL)膜在三维软骨再生中的潜在优势。然而，GT/PCL比值的变化是否对三维软骨再生有显著影响尚不清楚。为了解决这一问题，本研究制备了三种不同GT/PCL比例(70：30，50：50，30：70)的电纺膜。观察软骨细胞在复合膜上的黏附和增殖情况，评价复合膜的生物相容性。设计不同三维形态的软骨，进一步评价GT/PCL比例对软骨再生的影响。结果表明，不同GT/PCL比例的复合膜与软骨细胞具有良好的生物相容性。然而，在体内3周时，膜中高含量的PCL显著阻碍了早期三维软骨的形成。出乎意料的是，在12周时，所有的圆柱形结构都形成了成熟的软骨样组织，组间没有统计学差异。令我们惊讶的是，耳形软骨的再生又获得了完全不同的结果：高含量的PCL在12周时就完全破坏了软骨的再生，只有最少的PCL含量组形成了均匀连续的软骨，其形状和弹性与人耳相似。这些结果表明，较高的PCL含量不利于三维软骨的再生，尤其是形状复杂的软骨，GT/PCL的比例为70：30可能是较合适的耳形软骨再生比例。本研究中建立的研究模型为基于电纺GT/PCL膜的软骨和其他组织再生提供了详细的信息。(C)2013爱思唯尔有限公司。保留所有权利。
Scaffolds play an important role in directing three-dimensional (3-D) cartilage regeneration. Our recent study reported the potential advantages of electrospun gelatin/polycaprolactone (GT/PCL) membranes in regenerating 3-D cartilage. However, it is still unknown whether the changes of GT/PCL ratio have significant influence on 3-D cartilage regeneration. To address this issue, the current study prepared three kinds of electrospun membranes with different GT/PCL ratios (70:30, 50:50, 30:70). Adhesion and proliferation of chondrocytes on the membranes were examined to evaluate biocompatibility of the membranes. Cartilage with different 3-D shapes was engineered to further evaluate the influences of GT/PCL ratio on cartilage regeneration. The current results demonstrated that all the membranes with different GT/PCL ratios presented good biocompatibility with chondrocytes. Nevertheless, the high PCL content in the membranes significantly hampered early 3-D cartilage formation at 3 weeks in vivo. Unexpectedly, at 12 weeks, all the cylinder-shaped constructs formed mature cartilage-like tissue with no statistical differences among groups. To our surprise, ear-shaped cartilage regeneration obtained quite different results again: the high PCL content completely disrupted cartilage regeneration even at 12 weeks, and only the least PCL content group formed homogeneous and continuous cartilage with a satisfactory shape and elasticity similar to human ear. All these results indicated that the high PCL content was unfavorable for 3-D cartilage regeneration, especially for the cartilage with a complicated shape, and that GT/PCL 70:30 might be a relatively suitable ratio for ear-shaped cartilage regeneration. The research models established in the current study provide detailed information for cartilage and other tissue regeneration based on electrospun GT/PCL membranes. (C) 2013 Elsevier Ltd. All rights reserved.