Genomewide analysis of gene expression in Staphylococcus epidermidis biofilms:: Insights into the pathophysiology of S-epidermidis biofilms and the role of phenol-soluble modulins in formation of biofilms
Genomewide analysis of gene expression in Staphylococcus epidermidis biofilms:: Insights into the pathophysiology of S-epidermidis biofilms and the role of phenol-soluble modulins in formation of biofilms
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DOI：
10.1086/426945
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发表时间：
2005-01-15
影响因子：
6.4
通讯作者：
Otto, M
中科院分区：
文献类型：
--
作者：
Yao, YF;Sturdevant, DE;Otto, M
许多细菌病原体形成被称为生物膜的细胞团聚体，这极大地限制了抗生素治疗和人体免疫防御的成功。为了深入了解主要的医院病原体表皮葡萄球菌的病理生理学，我们分析了形成生物膜的表皮葡萄球菌的基因组，构建了代表其整个转录组的微阵列，并对表皮葡萄球菌生物膜进行了表达谱分析。生物膜中的基因调控过程导致细菌以非攻击性和受保护的形式生长，代谢活性低，最适合保证慢性感染期间的长期存活。一类被称为酚溶性调节蛋白的肽，将促炎活性与生物膜分离的推定作用结合起来，演变为控制侵袭性和静止感染模式之间转换的潜在关键决定因素。我们的数据表明，表皮葡萄球菌通过基因表达的广泛变化来调整其生活方式，以适应定植和感染期间的不同需求。观察到的生物膜生长模式的生理特征，特别是表面活性剂样肽的贡献，可以作为多种生物膜形成病原体的模型。
Many bacterial pathogens form cellular agglomerations known as biofilms, which considerably limit the success of both antibiotic treatment and the human immune defense. To gain insight into the pathophysiology of the leading nosocomial pathogen, Staphylococcus epidermidis, we analyzed the genome of biofilm-forming S. epidermidis, constructed a microarray representing its entire transcriptome, and performed expression profiling of an S. epidermidis biofilm. Gene-regulated processes in the biofilm led to a nonaggressive and protected form of bacterial growth with low metabolic activity, which is optimally suited to guarantee long-term survival during chronic infection. A class of peptides known as phenol-soluble modulins, which combine proinflammatory activity with a putative role in detachment of biofilms, evolved as potential key determinants controlling the switch between aggressive and quiescent modes of infection. Our data suggest that S. epidermidis adjusts its lifestyle to varying requirements during colonization and infection by means of an expansive change of gene expression. The observed physiological characteristics of the biofilm mode of growth-in particular, the contribution of surfactant-like peptides-might serve as a model for a variety of biofilm-forming pathogens.