Genome-wide identification and characterization of odorant-binding protein (OBP) genes in the malaria vector Anopheles sinensis (Diptera: Culicidae)
Genome-wide identification and characterization of odorant-binding protein (OBP) genes in the malaria vector Anopheles sinensis (Diptera: Culicidae)
复制标题
疟疾载体中华按蚊（双翅目：蚊科）中气味结合蛋白（OBP）基因的全基因组鉴定和表征
DOI：
10.1111/1744-7917.12333
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发表时间：
2016-06-01
期刊：
影响因子：
4
通讯作者：
Chen, Bin
中科院分区：
文献类型：
--
作者：
He, Xiu;He, Zheng-Bo;Chen, Bin
中华按蚊是一种主要的疟疾传播媒介。昆虫气味结合蛋白（OBPs）可能在嗅觉系统中参与气味物质的接收。目前，对于中华按蚊OBP基因的分类和特性尚未进行系统研究。在本研究中，基于OBP保守基序PBP_GOBP之间的比较，以及与冈比亚按蚊OBPs的系统发育分析，在中华按蚊全基因组水平上鉴定出64个推定的OBP基因。对中华按蚊OBPs的特性，包括基序保守性、基因结构、基因组组织和分类进行了研究。在转录本和保守基序的支持下，鉴定出一个属于Plus-C亚家族的新基因AsOBP73。这些中华按蚊OBP基因被分为三个亚家族，其中经典亚家族有37个基因，非典型亚家族有15个基因，Plus-C亚家族有12个基因。中华按蚊OBPs的基因组组织表明，它们在九个不同的支架上呈簇状分布。八个基因（OBP23 - 28、OBP63、OBP64）可能至少在按蚊、库蚊和伊蚊分化之前，通过一系列历史上的复制事件从单个基因演化而来。微观共线性分析表明，中华按蚊和冈比亚按蚊的OBPs之间具有很高的共线性。在本研究中，之前在冈比亚按蚊中归类于Plus-C亚家族的OBP70和OBP71被认定属于经典亚家族的Obp59a组，而之前归类于Plus-C亚家族的OBP69被重新划分到非典型亚家族。本研究为进一步研究昆虫尤其是中华按蚊的OBP基因建立了基本信息框架。
Anopheles sinensis is a major malaria vector. Insect odorant-binding proteins (OBPs) may function in the reception of odorants in the olfactory system. The classification and characterization of the An. sinensis OBP genes have not been systematically studied. In this study, 64 putative OBP genes were identified at the whole-genome level of An. sinensis based on the comparison between OBP conserved motifs, PBP_GOBP, and phylogenetic analysis with An. gambiae OBPs. The characterization of An. sinensis OBPs, including the motif's conservation, gene structure, genomic organization and classification, were investigated. A new gene, AsOBP73, belonging to the Plus-C subfamily, was identified with the support of transcript and conservative motifs. These An. sinensis OBP genes were classified into three subfamilies with 37, 15 and 12 genes in the subfamily Classic, Atypical and Plus-C, respectively. The genomic organization of An. sinensis OBPs suggests a clustered distribution across nine different scaffolds. Eight genes (OBP23-28, OBP6364) might originate from a single gene through a series of historic duplication events at least before divergence of Anopheles, Culex and Aedes. The microsynteny analyses indicate a very high synteny between An. sinensis and An. gambiae OBPs. OBP70 and OBP71 earlier classified under Plus-C in An. gambiae are recognized as belonging to the group Obp59a of the Classic subfamily, and OBP69 earlier classified under Plus-C has been moved to the Atypical subfamily in this study. The study established a basic information frame for further study of the OBP genes in insects as well as in An. sinensis.