The alpha 1-alpha 6 subunits of integrins are characteristically expressed in distinct segments of developing and adult human nephron.
The alpha 1-alpha 6 subunits of integrins are characteristically expressed in distinct segments of developing and adult human nephron.
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整联蛋白的α1-α6亚基特征是在发展中和成人人类肾脏的不同段中表达。
DOI：
10.1083/jcb.111.3.1245
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发表时间：
1990-09
期刊：
影响因子：
--
通讯作者：
Virtanen I
中科院分区：
文献类型：
--
作者：
Korhonen M;Ylänne J;Laitinen L;Virtanen I
我们用一组单抗在间接免疫荧光显微镜下研究了β1整合素的α1-α6亚基在发育和成人肾脏中的分布。未诱导间充质仅对α1整合素亚单位呈弥漫性免疫反应。在肾单位发育的S形体阶段，几个α亚基在不同的胎儿肾单位节段中有特征性的表达，这种模式在成人肾单位中也保留了下来。因此，α1亚基在系膜细胞和内皮细胞中有特征性的表达，α2在肾小球内皮细胞和远曲小管中表达，α3在足细胞、鲍曼囊和远曲小管中表达，而α6亚基基本在所有的小管中表达，在发育过程中仅在足细胞中短暂表达。与α3和α6亚基不同，α2亚基在远端小管中显示出整体的细胞表面分布。在肾小球形成过程中，其在肾小球内皮细胞也有明显表达。β4亚基仅在胎儿集合管中表达，因此在人肾中，α6亚基似乎与β1而不是β4亚基形成复合体。在纤维连接蛋白受体α4和α5两个亚基中，仅有后者表达，仅局限于发育和成人血管内皮细胞，提示这些受体复合体在肾脏形成过程中起着较小的作用。目前的结果表明，不同的整合素在特定肾单位节段的分化过程中发挥了作用。它们还表明，α3β1和α6β1整合素复合体可能是足细胞和肾小管上皮细胞的基底膜受体。
We studied the distribution of the alpha 1-alpha 6 subunits of beta 1 integrins in developing and adult human kidney using a panel of mAbs in indirect immunofluorescence microscopy. Uninduced mesenchyme displayed a diffuse immunoreactivity for only the alpha 1 integrin subunit. At the S-shaped body stage of nephron development, several of the alpha subunits were characteristically expressed in distinct fetal nephron segments, and the pattern was retained also in the adult nephron. Thus, the alpha 1 subunit was characteristically expressed in mesangial and endothelial cells, the alpha 2 in glomerular endothelium and distal tubules, the alpha 3 in podocytes, Bowman's capsule, and distal tubules, and the alpha 6 subunit basally in all tubules, and only transiently in podocytes during development. Unlike the alpha 3 and alpha 6 subunits, the alpha 2 subunit displayed an overall cell surface distribution in distal tubules. It was also distinctly expressed in glomerular endothelia during glomerulogenesis. The beta 4 subunit was expressed only in fetal collecting ducts, and hence the alpha 6 subunit seems to be complexed with the beta 1 rather than beta 4 subunit in human kidney. Of the two fibronectin receptor alpha subunits, alpha 4 and alpha 5, only the latter was expressed, confined to endothelia of developing and adult blood vessels, suggesting that these receptor complexes play a minor role during nephrogenesis. The present results suggest that distinct integrins play a role during differentiation of specific nephron segments. They also indicate that alpha 3 beta 1 and alpha 6 beta 1 integrin complexes may function as basement membrane receptors in podocytes and tubular epithelial cells.