Isolation and characterization of hyper phenol tolerant Bacillus sp from oil refinery and exploration sites
Isolation and characterization of hyper phenol tolerant Bacillus sp from oil refinery and exploration sites
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DOI：
10.1016/j.jhazmat.2009.11.002
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发表时间：
2010-04-15
影响因子：
13.6
通讯作者：
Ghoshal, Aloke K.
中科院分区：
文献类型：
--
作者：
Banerjee, Aditi;Ghoshal, Aloke K.
分别从炼油厂和石油勘探现场分离到蜡状芽孢杆菌MTCC 9817菌株AKG1和蜡状芽孢杆菌MTCC 9818菌株AKG2。菌株AKG1的16S rDNA序列与蜡样芽孢杆菌的同源性为99.63%，与凝结芽孢杆菌的同源性为99.63%，与苏云金芽孢杆菌2PR56-10的同源性为99.34%。AKG2与苏云金芽胞杆菌CMG 861株的同源性为99.37%。AKG1和AKG2之间的相似性搜索给出了同属相似序列中最低的99.19%。在苯酚浓度为1000 mg/L时，AKG1的最适生长条件为37℃，最适pH为7.0，AKG2的最适生长条件为37℃，最适pH为7.5。菌株AKG1和AKG2的生长动力学分别符合Yano模型(最大生长速率，m u(Max)=1.024 h(-1))和爱德华模型(m u(Max)=0.5969 h(-1)，K-L=1 483 mg/L)。AKG1m(Max)=637.8 mg/L，K-L=637.8 mg/L，K-L=494.4 mg/L，m(Max)=0.9332 h(-1)，符合霍尔丹模型。(C)2009爱思唯尔B.V.保留所有权利。
Bacillus cereus MTCC 9817 strain AKG1 and B. cereus MTCC 9818 strain AKG2 were isolated from petroleum refinery and oil exploration site, respectively. The 16S rDNA sequence of strain AKG1 showed the closest relation to B. cereus 99.63% and Bacillus coagulans 99.63% followed by 99.34% homology with Bacillus thuringiensis strain 2PR56-10. AKG2 is mostly related to B. thuringiensis strain CMG 861 with 99.37% homology. The similarity search between AKG1 and AKG2 gave the lowest similarity 99.19% among same genus similar sequences. At phenol concentration of 1000 mg/L, the optimum growth conditions for AKG1 were found to be 37 degrees C and pH 7.0 and the same were found to be 37 degrees C and pH 7.5 for AKG2. The growth kinetics of the strains AKG1 and AKG2 are best fitted by Yano model (maximum growth rate, mu(max) = 1.024 h(-1) and inhibition constant, K-l = 171,800 mg/L) and Edward model (mu(max) = 0.5969 h(-1) and K-l = 1483 mg/L) respectively. Growth kinetics of both the strains are also well fitted by the Haldane model with mu(max) = 0.4396 h(-1) and K-l = 637.8 mg/L for AKG1 and mu(max) = 0.9332 h(-1) and K-l = 494.4 mg/L for AKG2. (C) 2009 Elsevier B.V. All rights reserved.