Genetic diversity and potential function of microbial symbionts associated with newly discovered species of Osedax polychaete worms
Genetic diversity and potential function of microbial symbionts associated with newly discovered species of Osedax polychaete worms
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DOI：
10.1128/aem.01986-06
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发表时间：
2007-04-01
影响因子：
4.4
通讯作者：
Vrijenhoek, Robert C.
中科院分区：
文献类型：
--
作者：
Goffredi, Shana K.;Johnson, Shannon B.;Vrijenhoek, Robert C.
我们调查了与来自加利福尼亚州蒙特雷峡谷的两种新发现的Osedax物种相关的共生细菌的遗传多样性，深度为1,017米（Osedax蒙特雷湾物种3“玫瑰色”[Osedax物种MB 3]）和381米（Osedax蒙特雷湾物种4“黄领”）。16 S rRNA基因序列的定量PCR和克隆文库确定了与新发现的宿主物种相关的细菌种类的组成和丰度的差异，并允许在成年Osedax frankpressi和最近在2，891米处植入的鲸骨中定居的青少年之间进行比较。新发现的Osedax物种承载了Oceanoalbellales共生体，这些共生体与之前描述的O. frankpressi和红纹刺蛾Osedax rubipumus（S. K. Goffredi，V. J. Orphan，G. W.劳斯湖Jahnke，T. Embaye，K.特克河Lee和R. C.弗里延胡克Microbiol. 7：1369-1378，2005）。此外，Osedax sp. MB 3宿主以ε变形菌门为主的其他细菌的多样性和丰富的群体。共生体承载根组织的超微结构分析验证了Osedax sp. MB 3的微生物多样性增强。新发现的寄主植物和O. frankpressi都表现出溶胶原酶活性，这与共生体DNA的丰度呈正相关，与宿主物种的平均成虫大小呈负相关。这种不寻常的食骨蠕虫属的成员可能与共生细菌形成可变的联系，从而在世界各地的海洋中观察到鲸鱼坠落环境中殖民化和成功的差异。
We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene sequences identified differences in the compositions and abundances of bacterial phylotypes associated with the newly discovered host species and permitted comparisons between adult Osedax frankpressi and juveniles that had recently colonized whalebones implanted at 2,891 m. The newly discovered Osedax species hosted Oceanospirillales symbionts that are related to Gammaproteobacteria associated with the previously described O. frankpressi and Osedax rubiplumus (S. K. Goffredi, V. J. Orphan, G. W. Rouse, L. Jahnke, T. Embaye, K. Turk, R. Lee, and R. C. Vrijenhoek, Environ. Microbiol. 7:1369-1378, 2005). In addition, Osedax sp. MB3 hosts a diverse and abundant population of additional bacteria dominated by Epsilonproteobacteria. Ultrastructural analysis of symbiont-bearing root tissues verified the enhanced microbial diversity of Osedax sp. MB3. Root tissues from the newly described host species and O. frankpressi all exhibited collagenolytic enzyme activity, which covaried positively with the abundance of symbiont DNA and negatively with mean adult size of the host species. Members of this unusual genus of bone-eating worms may form variable associations with symbiotic bacteria that allow for the observed differences in colonization and success in whale fall environments throughout the world's oceans.