Yeast as a tool to study Bax/mitochondrial interactions in cell death.
Yeast as a tool to study Bax/mitochondrial interactions in cell death.
复制标题
酵母作为研究细胞死亡中 Bax/线粒体相互作用的工具。
DOI：
10.1016/s1567-1356(03)00143-0
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发表时间：
2003
影响因子：
3.2
通讯作者：
Manon,Stéphen
中科院分区：
文献类型：
--
作者：
Priault,Muriel;Camougrand,Nadine;Kinnally,KathleenW;Vallette,FrançoisM;Manon,Stéphen
萌芽酵母已被证明是研究与细胞凋亡有关的分子方面的一个强有力的工具，特别是涉及线粒体的步骤。酵母对参与细胞凋亡调控的蛋白质没有明显的同源性，并提供了一个简化的模型系统，在这个系统中这些蛋白质的功能可以被解开。本文就两个主要的促凋亡基因Bax和Bid与线粒体的相互作用作一综述。结果表明，酵母允许质疑这两种蛋白质功能的几个关键方面，即驱动它们插入线粒体外膜的分子机制和导致细胞色素通透性的分子机制。最近，在酵母中发现了导致Bax诱导的细胞死亡以及其他形式的细胞死亡的信号通路。在这两条信号通路中，线粒体都扮演着核心角色，这两种形式的细胞降解都与类凋亡和自噬有关。
The budding yeastSaccharomyces cerevisiaehas proven to be a powerful tool in investigations of the molecular aspects of the events involved in apoptosis, particularly the steps implicating mitochondria. Yeast does not have obvious homologs of the proteins involved in the regulation of apoptosis, and provides a simplified model system in which the function of these proteins can be unraveled. This review focuses on the interactions of two of the major pro-apoptotic Bcl-2 family members, Bax and Bid, with mitochondria. It is shown that yeast has allowed questioning of several crucial aspects of the function of these two proteins, namely the molecular mechanisms driving their insertion into the mitochondrial outer membrane and those leading to the permeabilization to cytochromec. More recently, signaling pathways leading to Bax-induced cell death, as well as other forms of cell death, have been identified in yeast. Both ‘apoptosis-like’ and autophagy-related forms of cell degradation are involved, and mitochondria play a central role in these two signaling pathways.