[Endogenous androgen levels of human prostatic tissues].
[Endogenous androgen levels of human prostatic tissues].
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[人前列腺组织的内源性雄激素水平]。
DOI：
10.1507/endocrine1927.58.7_876
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
A. Kanbegawa
中科院分区：
文献类型：
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作者：
M. Morioka;K. Takeda;N. Mitsuhata;T. Ohashi;H. Ohmori;T. Saito;A. Kanbegawa
使用Sephadex LH-20柱色谱法通过放射免疫测定法测定良性前列腺肥大（BPH）和未经治疗的前列腺癌（PC）患者前列腺组织的内源性雄激素水平。通过该测定程序，仅用 30 个约 40 mg 重量的组织就可以测量组织雄激素水平。在 BPH (n=20) 中，类固醇浓度（平均值 +/- 标准偏差，ng/g. 组织重量）为 5 α-雄甾烷 3 α、17 β 二醇 (A-二醇)，2.31 +/- 1.29；二氢睾酮 (DHT)，4.97 +/- 2.17；睾酮 (T)，0.62 +/- 0.33。在 PC (n=17) 中，浓度为 5.51 +/- 3.23； 3.94+/-3.16；和0.93+/-0.38；分别。与PC相比，BPH中DHT浓度显着升高，A-二醇水平显着降低（P小于0.01）。 PC 的组织 DHT 水平各不相同，但可分为两组。一种低于 2 ng 水平 (n=8)，与非雄激素靶组织相似，另一种高于 2 ng (n=19)。研究了前列腺癌组织学分化和组织 DHT 水平之间的关系。在低分化癌症（n=4）中，所有病例的 DHT 水平都很低，而且即使在高分化癌症中，也有 4 例的水平低于 2 ng。因此，前列腺组织内源性DHT水平的测量被认为对于判断前列腺癌雄激素依赖性具有重要价值。
Endogenous androgen levels of prostatic tissues in patients with benign prostatic hypertrophy (BPH) and untreated prostatic cancer (PC) were determined by radioimmunoassay using sephadex LH-20 column chromatography. By this assay procedure, it was possible to measure tissue androgen levels with only 30 approximately 40 mg weight of tissues. In BPH (n=20) the concentrations of steroids (mean +/- standard deviation, ng/g. tissue weight) were 5 alpha-androstane 3 alpha, 17 beta diol (A-diol), 2.31 +/- 1.29; dihydrotestosterone (DHT), 4.97 +/- 2.17; testosterone (T), 0.62 +/- 0.33. In PC (n=17) the concentrations were 5.51 +/- 3.23; 3.94 +/- 3.16; and 0.93 +/- 0.38; respectively. The concentrations of DHT were raised and the levels of A-diol were reduced significantly (P less than 0.01) in BPH compared with PC. Tissue DHT levels of PC were variable, but they could be classified in to two groups. One was below 2 ng levels (n=8) similar to those of non-androgen target tissues and the other was over 2 ng (n=19). The relationships between histological differentiation and tissue DHT levels in prostatic cancer were examined. In poorly differentiated cancer (n=4), DHT levels were low in all cases, furthermore even in well differentiated ones there were 4 cases with levels below 2 ng. Thus, the measurement of endogenous DHT levels of prostatic tissues was considered to be valuable for the judgment of androgen dependency in prostatic cancer.