Characterization of the ABA-deficient tomato mutant notabilis and its relationship with maize Vp14
Characterization of the ABA-deficient tomato mutant notabilis and its relationship with maize Vp14
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DOI：
10.1046/j.1365-313x.1999.00386.x
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发表时间：
1999-02-01
期刊：
影响因子：
7.2
通讯作者：
Taylor, IB
中科院分区：
文献类型：
--
作者：
Burbidge, A;Grieve, TM;Taylor, IB
番茄notabilis(非)突变体由于缺乏植物激素脱落酸(ABA)水平而具有枯萎表型。突变体似乎在ABA生物合成的关键控制步骤中存在缺陷-9-顺式叶黄素前体的氧化裂解形成C-15中间体花椒毒素。最近通过转座子诱变获得了一个玉米突变体--胎生型14(Vp14)。这种玉米遗传损伤也影响了ABA合成中的氧化裂解步骤。根据预测的VP14氨基酸序列设计了简并的聚合酶链式反应引物，为筛选番茄枯萎病相关基因文库提供了探针。获得了一个针对NOT基因的全长基因克隆。番茄和玉米Vp14基因的开放阅读框(ORF)除了部分N端序列外，都非常相似。NOT突变已经在DNA水平上得到了表征。编码序列的特定A/T碱基对缺失导致了移码突变，表明NOT是一个空突变体。这一观察结果与非突变纯合子表现出的相对温和的表型有关。
The notabilis (not) mutant of tomato has a wilty phenotype due to a deficiency in the levels of the plant hormone abscisic acid (ABA). The mutant appears to have a defect in a key control step in ABA biosynthesis - the oxidative cleavage of a 9-cis xanthophyll precursor to form the C-15 intermediate, xanthoxin. A maize mutant, viviparous 14 (vp14) was recently obtained by transposon mutagenesis. This maize genetic lesion also affects the oxidative cleavage step in ABA synthesis. Degenerate primers for PCR, based on the VP14 predicted amino acid sequence, have been used to provide probes for screening a wilt-related tomato cDNA library. A full-length cDNA clone was identified which is specific to the not gene locus. The ORFs of the tomato cDNA and maize Vp14 are very similar, apart from parts of their N-terminal sequences. The not mutation has been characterized at the DNA level. A specific A/T base pair deletion of the coding sequence has resulted in a frameshift mutation, indicating that not is a null mutant. This observation is discussed in connection with the relatively mild phenotype exhibited by not mutant homozygotes.