On the structure of heparitin sulfates. Analyses of the products formed from heparitin sulfates by two heparitinases and a heparinase from Flavobacterium heparinum.
On the structure of heparitin sulfates. Analyses of the products formed from heparitin sulfates by two heparitinases and a heparinase from Flavobacterium heparinum.
复制标题
硫酸肝素的结构。
DOI：
10.1016/0304-4165(76)90354-8
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
H. Nader
中科院分区：
文献类型：
--
作者：
M. Silva;C. Dietrich;H. Nader
本文报道了肝素硫酸盐在两种肝素酶和一种肝素黄杆菌酶作用下的产物分析。肝素硫酸盐A和B被肝素酶I降解，产生两个双糖，一个由N-乙酰氨基葡萄糖和一个不饱和糖醛酸组成，通过α(1→4)键连接，另一个组成相同，但在己糖胺部分有ANO-硫酸盐。第三种二糖也是由硫酸肝素B在同一种酶的作用下形成的，由氨基葡萄糖N-硫酸盐和可能通过α(1→4)键连接的不饱和糖醛酸组成。除了这三种二糖外，硫酸肝素B在肝素酶I的作用下产生一种寡糖(平均分子量为6000)，它被肝素酶II完全降解，生成由氨基葡萄糖、2，6-二硫酸盐和不饱和糖醛酸组成的二糖。肝素黄杆菌的α-葡聚糖苷酶进一步降解所有二糖，产生相应的单糖。基于这些分析和其他分析，提出了肝素硫酸盐的可能结构。
The analyses of the products formed from heparitin sulfates by the action of two heparitinases and a heparinase fromFlavobacterium heparinumis reported. Heparitin sulfates A and B are degraded by heparitinase I yielding two disaccharides, one of them composed ofN-acetylglucosamine and an unsaturated uronic, joined byα(1 → 4) linkage, and the other, with the same composition but with anO-sulfate at the hexosamine moiety. A third disaccharide is also formed from heparitin sulfate B, by the action of the same enzyme, composed of glucosamineN-sulfate and an unsaturated uronic acid joined probably byα(1 → 4) linkage. Besides these three disaccharides, heparitin sulfate B yields, by the action of heparitinase I, an oligosaccharide (with an average molecular weight of 6000) which is completely degraded by the heparitinase II yielding a disaccharide composed of glucosamine 2,6-disulfate and unsaturated uronic acid. All the disaccharides are further degraded by α-glycuronidase fromFlavobacterium heparinumyielding the respective monosaccharides. Based on these and other analyses the possible structures of the heparitin sulfates are proposed.