Wireless Force-Inducing Neuronal Stimulation Mediated by High Magnetic Moment Microdiscs.
Wireless Force-Inducing Neuronal Stimulation Mediated by High Magnetic Moment Microdiscs.
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DOI：
10.1002/adhm.202101826
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发表时间：
2022-03
影响因子：
10
通讯作者：
Romero, Gabriela
中科院分区：
文献类型：
--
作者：
Collier, Claudia;Muzzio, Nicolas;Thevi Guntnur, Rohini;Gomez, Amanda;Redondo, Carolina;Zurbano, Raquel;Schuller, Ivan K.;Monton, Carlos;Morales, Rafael;Romero, Gabriela
关键词：
细胞信号传导的非侵入性操纵对于基础神经科学研究以及开发神经系统疾病和精神疾病的疗法至关重要。在这里，无线力诱导的初级神经元回路的刺激，通过机械转导介导的磁性微盘（MMD）下施加的低强度和低频交变磁场（AMF），进行了描述。MMD通过自上而下的光刻技术制造，其允许具有高饱和度和零剩磁磁矩的生物相容性MMD的具有成本效益的大规模生产。MMD被用作AMF到机械力的换能器。当MMD暴露于原代大鼠神经元回路时，它们的磁-机械致动触发细胞膜上表达的特定机械敏感离子通道的响应，激活接受治疗的海马神经元的约50%和皮质神经元的约90%。通过用Gd 3+抑制机械敏感性跨膜通道来证实机械转导。由MMD介导的机械转导对神经元培养物没有细胞毒性作用。该技术满足了细胞类型特异性和弱磁场的要求，这是非侵入性神经调节疗法和临床设备设计开发中的两个限制因素。此外，还成功地实现了高效率和持久的刺激。这项研究代表了使用具有定制磁特性的盘形微材料进行神经活动的磁机械控制的基本步骤。
Noninvasive manipulation of cell signaling is critical in basic neuroscience research and in developing therapies for neurological disorders and psychiatric conditions. Here, the wireless force-induced stimulation of primary neuronal circuits through mechanotransduction mediated by magnetic microdiscs (MMDs) under applied low-intensity and low-frequency alternating magnetic fields (AMFs), is described. MMDs are fabricated by top-down lithography techniques that allow for cost-effective mass production of biocompatible MMDs with high saturation and zero magnetic magnetic moment at remanence. MMDs are utilized as transducers of AMFs into mechanical forces. When MMDs are exposed to primary rat neuronal circuits, their magneto-mechanical actuation triggers the response of specific mechanosensitive ion channels expressed on the cell membranes activating ≈50% of hippocampal and ≈90% of cortical neurons subjected to the treatment. Mechanotransduction is confirmed by the inhibition of mechanosensitive transmembrane channels with Gd3+. Mechanotransduction mediated by MMDs cause no cytotoxic effect to neuronal cultures. This technology fulfills the requirements of cell-type specificity and weak magnetic fields, two limiting factors in the development of noninvasive neuromodulation therapies and clinical equipment design. Moreover, high efficiency and long-lasting stimulations are successfully achieved. This research represents a fundamental step forward for magneto-mechanical control of neural activity using disc-shaped micromaterials with tailored magnetic properties.
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影响因子：
2
作者：
Georgiou, Anastasios M.;Lada, Eleni;Kambanaros, Maria
通讯作者：
Kambanaros, Maria
影响因子：
10.6
作者：
Dinur-Klein, Limor;Dannon, Pinhas;Zangen, Abraham
通讯作者：
Zangen, Abraham
影响因子：
4.6
作者：
Arenas-Guerrero P;Delgado ÁV;Donovan KJ;Scott K;Bellini T;Mantegazza F;Jiménez ML
通讯作者：
Jiménez ML
影响因子：
3.9
作者：
Badaire, S;Poulin, P;Zakri, C
通讯作者：
Zakri, C
DOI：
10.2214/ajr.14.13632
发表时间：
2015-07
期刊：
AJR. American journal of roentgenology
影响因子：
--
作者：
Ghanouni P;Pauly KB;Elias WJ;Henderson J;Sheehan J;Monteith S;Wintermark M
通讯作者：
Wintermark M