Polymorphisms of cytochrome b gene in Leishmania parasites and their relation to types of cutaneous leishmaniasis lesions in Pakistan
Polymorphisms of cytochrome b gene in Leishmania parasites and their relation to types of cutaneous leishmaniasis lesions in Pakistan
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DOI：
10.1111/j.1346-8138.2008.00419.x
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发表时间：
2008-02-01
影响因子：
3.1
通讯作者：
Uezato, Hiroshi
中科院分区：
文献类型：
--
作者：
Myint, Chomar Kaung;Asato, Yutaka;Uezato, Hiroshi
涉及的利什曼原虫的确切种属和/或菌株强烈影响利什曼病的临床和流行病学特征，而目前对这些影响和关系的认识是不够的。我们报告，细胞色素B（cyt B）基因测序确定致病利什曼原虫寄生虫的69皮肤利什曼病病例在巴基斯坦超过3年的时间。高原地区（俾路支省、奎达市）21例，经鉴定为利什曼原虫16例（76.2%）。热带利什曼原虫5株（23.8%）。少校在来自信德省和俾路支省的低地地区、印度河流域的城市/村庄的48例病例中，47例（97.9%）被鉴定为L.（L.）major和L.（L.）热带。统计分析（Fisher精确检验）显示两种鸟类在海拔高度上的分布有显著差异（P < 0.0001）;（L.）major在低地和L.（L.）高原地区的热带。本研究结果丰富了我们早期的发现，即根据第一年的培养寄生虫数据，只有L。（L.）tropica分布于高原地区，仅L.（L.）主要分布在低地地区。在所分析的利什曼原虫样本中，存在利什曼原虫cyt B多态性的3种类型。（L.）其中Ⅰ型45例（86.5%），Ⅱ型6例（11.5%），Ⅲ型1例（2%）。我们首次报道了L.（L.）主要（I型、II型和III型），基于使用来自临床样品的cyt B基因测序的种属鉴定。此外，我们发现临床表现（湿，干和/或混合型皮肤病变）和致病利什曼原虫寄生虫之间没有相关性。
The exact species and/or strains of Leishmania parasites involved strongly influence the clinical and epidemiological features of leishmaniasis, and current knowledge of those influences and relationships is inadequate. We report that cytochrome b (cyt b) gene sequencing identified causal Leishmania parasites of 69 cutaneous leishmaniasis cases in Pakistan over a 3-year period. Of 21 cases in highland areas (Quetta city, Balochistan province), 16 (76.2%) were identified as Leishmania (L.) tropica and five (23.8%) as Leishmania (L.) major. Of 48 cases from lowland areas, cities/villages in Indus valley in Sindh and Balochistan provinces, 47 (97.9%) were identified as L. (L.) major and one (2.1%) as L. (L.) tropica. Statistical analysis (Fisher's exact test) revealed a significant difference (P < 0.0001) in the distribution of the two species by altitude; L. (L.) major is predominant in lowland and L. (L.) tropica at highland areas. The present result enriched our earlier finding, based on the first year's cultured parasite data, that only L. (L.) tropica was found in highland areas and only L. (L.) major in lowland areas. Among Leishmania samples analyzed, three types of cyt b polymorphism of L. (L.) major were found, including 45 (86.5%) cases of type I, six (11.5%) of type II and one (2%) of type III. We report for the first time on the presence of polymorphisms in L. (L.) major (types I, II and III) based on species identification using cyt b gene sequencing from clinical samples. Moreover, we found no correlation between clinical presentation (wet-, dry- and/or mixed-types of cutaneous lesions) and causal Leishmania parasites.