Conjugated polyelectrolytes-initiated chemiluminescence: A biosensing platform for label-free and homogeneous DNA detection
Conjugated polyelectrolytes-initiated chemiluminescence: A biosensing platform for label-free and homogeneous DNA detection
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共轭聚电解质引发的化学发光：用于无标记和同质 DNA 检测的生物传感平台
DOI：
10.1016/j.bios.2013.02.047
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发表时间：
2013-09-15
影响因子：
12.6
通讯作者：
Cui, Xiang
中科院分区：
文献类型：
--
作者：
Liu, Mei;Li, Baoxin;Cui, Xiang
研究发现，在弱碱性介质中，共轭聚电解质（CPE）能引发鲁米诺-H_2O_2体系的强化学发光。利用化学发光光谱、紫外可见光谱、荧光光谱和电子自旋共振（ESR）光谱等研究了CPE对Luminol-H2 O2化学发光的催化机理。此外，还发现聚[3-（3 '-N，N，N-三乙氨基-1'-丙氧基）-4-甲基-2，5-噻吩盐酸盐]（PMNT）（一种阳离子型CPE）对鲁米诺-H_2O_2化学发光体系的催化活性与PMNT的构象密切相关。鲁米诺-H_2O_2-PMNT体系在单链DNA存在下的化学发光强度远大于在双链DNA存在下的化学发光强度。利用这一现象，提出了一种无标记、均相的DNA杂交化学发光检测方法。靶DNA的检测限（3 σ）估计低至3.7 × 10（-13）M。该方法具有简便、廉价、快速、灵敏等优点。(C)2013爱思唯尔有限公司版权所有。
In this study, it was found that conjugated polyelectrolytes (CPEs) would initiate the strong chemiluminescence (CL) emission of luminol-H2O2 in weak basic media. Using CL spectra, ultraviolet visible light spectra, fluorescence spectra and electron spin resonance (ESR) spectra, the catalytic mechanism of CPEs on luminol-H2O2 CL was discussed in detail. Furthermore, it was found that the catalytic activity of poly [3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-thiophene hydro chloride] (PMNT) (a kind of cationic CPEs) on the luminol-H2O2 CL system was closely relevant to the conformation of PMNT. The CL intensity of luminol-H2O2-PMNT system in the presence of single-stranded DNA was much larger than that in the presence of double-stranded DNA. By taking advantage of this phenomenon, a label-free and homogeneous CL detection of DNA hybridization is proposed. The detection limit of target DNA (3 sigma) was estimated to be as low as 3.7x10(-13) M. The present CL method for DNA hybridization detection offers the advantages of being simple, cheap, rapid and sensitive. (C) 2013 Elsevier B.V. All rights reserved.