An aphid facultative symbiont suppresses plant defence by manipulating aphid gene expression in salivary glands
An aphid facultative symbiont suppresses plant defence by manipulating aphid gene expression in salivary glands
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蚜虫兼性共生体通过操纵唾液腺中的蚜虫基因表达来抑制植物防御
DOI：
10.1111/pce.13836
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sun Yucheng
中科院分区：
文献类型：
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作者：
Wang Qinyang;Yuan Erliang;Ling Xiaoyu;Zhu-Salzman Keyan;Guo Huijuan;Ge Feng;Sun Yucheng
蚜虫经常携带兼性共生体以获得多样化的优势。共生沙雷菌是兼性内共生菌之一，能提高蚜虫的耐热性。然而，它是否有利于蚜虫在寄主植物上定植还有待确定。在目前的研究中，我们发现了绿虹吸管。与无沙雷氏菌的蚜虫相比，共生蚜虫对短梗紫花苜蓿的取食时间更长。相比之下，与感染沙雷氏菌的蚜虫相比，无沙雷氏菌蚜虫引发了更高的活性氧（ROS）积累、茉莉酸和水杨酸应答基因以及胞质Ca2+升高。唾液腺的转录组学分析表明，富含组氨酸的Ca2+结合蛋白样基因（ApHRC）在感染沙雷氏菌的蚜虫的唾液腺中的表达高于未感染沙雷氏菌的蚜虫。一旦ceprrc1被沉默，感染沙雷氏菌的蚜虫也表现出更短的韧皮部摄食时间，并引起植物中Ca2+升高和ROS积累。我们的研究结果表明，aprc是一种受ys上调的潜在效应物。通过抑制Ca2+升高和ROS积累，使蚜虫定植，从而损害植物的防御反应。本研究揭示了兼性共生体如何促进蚜虫定植和适应寄主植物。
Aphids often carry facultative symbionts to achieve diverse advantages.Serratia symbiotica, one of facultative endosymbionts, increases aphid tolerance to heat. However, whether it benefits aphid colonization on host plants is yet to be determined. In the current study, we found thatAcyrthosiphon pisumharbouringS. symbioticahad longer feeding duration onMedicago truncatulathanSerratia‐free aphids. Contrastingly,Serratia‐free aphids triggered higher accumulation of reactive oxygen species (ROS), jasmonic acid and salicylic acid responsive genes and cytosolic Ca2+elevations thanSerratia‐infected aphids. Transcriptomic analysis of salivary glands indicated that a histidine‐rich Ca2+‐binding protein‐like gene (ApHRC) was expressed more highly in the salivary gland ofSerratia‐infected aphids than that ofSerratia‐free aphids. OnceApHRCwas silenced,Serratia‐infected aphids also displayed shorter phloem‐feeding duration and caused Ca2+elevation and ROS accumulation in plants. Our results suggest thatApHRC, a potential effector up‐regulated byS. symbioticain the salivary glands, impairs plant defence response by suppressing Ca2+elevation and ROS accumulation, allowing colonization of aphids. This study has provided an insight into how facultative symbionts facilitate aphid colonization and adaptation to host plants.