Live Imaging with Genetically Encoded Physiologic Sensors and Optogenetic Tools.
Live Imaging with Genetically Encoded Physiologic Sensors and Optogenetic Tools.
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DOI：
10.1016/j.jid.2022.12.002
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
S. Zaver;C. J. Johnson;Andre Berndt;C. Simpson
中科院分区：
文献类型：
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作者：
S. Zaver;C. J. Johnson;Andre Berndt;C. Simpson
屏障组织如表皮利用复杂的信号转导系统来执行形态发生程序，并对环境线索迅速作出反应以促进体内平衡。实时成像技术和工具的最新进展允许精确的空间和时间监测和操纵细胞内信号级联。利用天然存在的光敏蛋白质的化学性质，基因编码的荧光生物传感器已经成为可视化动态信号事件的强大工具。相比之下，光遗传学蛋白构建体允许活细胞、类器官甚至模型生物体内的信号受体和效应子的激光介导控制。在本文中，我们回顾了新型生物传感器和光遗传学工具的基本原理，并强调了皮肤生物学领域的最新研究如何利用这些成像策略来阐明调节表皮发育、屏障形成和组织稳态的时空信号。
Barrier tissues such as the epidermis employ complex signal transduction systems to execute morphogenetic programs and to rapidly respond to environmental cues to promote homeostasis. Recent advances in live-imaging techniques and tools allow precise spatial and temporal monitoring and manipulation of intracellular signaling cascades. Leveraging the chemistry of naturally occurring light-sensitive proteins, genetically encoded fluorescent biosensors have emerged as robust tools for visualizing dynamic signaling events. In contrast, optogenetic protein constructs permit laser-mediated control of signal receptors and effectors within live cells, organoids, and even model organisms. In this paper, we review the basic principles underlying novel biosensors and optogenetic tools and highlight how recent studies in cutaneous biology have leveraged these imaging strategies to illuminate the spatiotemporal signals regulating epidermal development, barrier formation, and tissue homeostasis.