Selective and stable elimination of endosymbionts from multiple‐infected whitefly Bemisia tabaci by feeding on a cotton plant cultured in antibiotic solutions
Selective and stable elimination of endosymbionts from multiple‐infected whitefly Bemisia tabaci by feeding on a cotton plant cultured in antibiotic solutions
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DOI：
10.1111/1744-7917.12703
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发表时间：
2020-10
期刊：
影响因子：
4
通讯作者：
Dongxiao Zhao;Zhi-chun Zhang;Hongtao Niu;Huifang Guo
中科院分区：
文献类型：
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作者：
Dongxiao Zhao;Zhi-chun Zhang;Hongtao Niu;Huifang Guo
母系可遗传的内共生菌具有多种生态系统功能。抗生素消除了特定的共生体，建立了缺乏某些共生体的实验宿主系，从而能够探索给定共生体的作用。中国烟粉虱有专性共生菌Portiera侵染个体，兼性共生菌如哈密尔顿、立克次体、卡氏菌等，混合感染较多。到目前为止，还没有研究评估在与不同共生体共感染的粉虱中使用抗生素消除特定共生体的选择性和有效性。此外，在建立某些无共生体的烟粉虱品系方面没有取得成功。本研究通过在含有利福平、氨苄西林或两者混合的水中培养的棉株饲养烟粉虱成虫，对感染烟粉虱品系、哈密尔顿-立克次体共侵染品系、哈密尔顿-立克次体共侵染品系和哈密尔顿-卡氏共侵染品系进行处理，旨在选择性地治疗烟粉虱共生菌感染，建立稳定的无共生菌品系。我们发现，氨苄西林选择性地消除卡氏杆菌而不影响波蒂埃拉、哈密尔顿氏菌和立克次体，尽管它们共存于同一宿主体内。同时，我们研究中考虑的所有共生菌都可以被利福平去除。在利福平治疗期间，兼性共生菌的减少比专性共生菌减少的速度要快得多。此外，我们还测量了烟粉虱在连续几代中消除共生菌的稳定性，并建立了自然种群中没有的立克次体感染株和卡氏杆菌感染株系。我们的结果为选择性消除共存于宿主中的共生菌和建立不同的共生菌感染宿主系提供了新的方案。
The maternally heritable endosymbiont provides many ecosystem functions. Antibiotic elimination of a specific symbiont and establishment of experimental host lines lacking certain symbionts enable the roles of a given symbiont to be explored. The whitefly Bemisia tabaci (Gennadius) in China harbors obligate symbiont Portiera infecting each individual, as well as facultative symbionts, such as Hamiltonella, Rickettsia and Cardinium, with co‐infections occurring relatively frequently. So far no studies have evaluated the selectivity and efficacy of a specific symbiont elimination using antibiotics in whiteflies co‐infected with different symbionts. Furthermore, no success has been achieved in establishing certain symbiont‐free B. tabaci lines. In this study, we treated Hamiltonella‐infected B. tabaci line, Hamiltonella‐Rickettsia‐co‐infected line and Hamiltonella‐Cardinium co‐infected line by feeding B. tabaci adults with cotton plants cultured in water containing rifampicin, ampicillin or a mixture of them, aiming to selectively curing symbiont infections and establishing stable symbiont‐free lines. We found ampicillin selectively eliminated Cardinium without affecting Portiera, Hamiltonella and Rickettsia, although they coexisted in the same host body. Meanwhile, all of the symbionts considered in our study can be removed by rifampicin. The reduction of facultative symbionts occurred at a much quicker pace than obligate symbiont Portiera during rifampicin treatment. Also, we measured the stability of symbiont elimination in whitefly successive generations and established Rickettsia‐infected and Cardinium‐infected lines which are absent in natural populations. Our results provide new protocols for selective elimination of symbionts co‐existing in a host and establishment of different symbiont‐infected host lines.