Ribotypes associated with Clostridium difficile outbreaks in Brazil display distinct surface protein profiles
Ribotypes associated with Clostridium difficile outbreaks in Brazil display distinct surface protein profiles
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DOI：
10.1016/j.anaerobe.2017.04.001
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发表时间：
2017-06-01
期刊：
影响因子：
2.3
通讯作者：
Ferreira, Eliane de Oliveira
中科院分区：
文献类型：
--
作者：
Ferreira, Thais Goncalves;Moura, Hercules;Ferreira, Eliane de Oliveira
艰难梭菌是一种形成芽胞的厌氧性肠道病原体，可引起艰难梭菌感染(CDI)。艰难梭菌是毒素介导的院内抗生素相关性腹泻的主要原因。CDI的发病机制是由两种主要的毒力因子TcdA和TcdB毒素引起的症状性感染。艰难梭菌还表达一些关键蛋白，包括细胞壁蛋白(CWPs)。S层蛋白(SLP)是一种CWP，它形成覆盖在细菌表面的准晶表面阵列。SLP在艰难梭菌与胃肠道的结合中起作用，但它们在毒力中的重要性需要更好地阐明。在这里，我们描述了从巴西的五个艰难梭菌临床分离株通过甘氨酸提取获得的表面浓缩蛋白质组分的自下而上的蛋白质组学分析，使用基于凝胶和无凝胶的方法。我们能够为每个菌株鉴定出大约250个蛋白质，其中包括S1pA、Cwp2、Cwp6、CwpV和Cwp84。已鉴定的CWP呈现不同的氨基酸覆盖率，这可能表明翻译后修饰的差异。对巴西艰难梭菌暴发的病原体--核型133的SLP进行的蛋白质组学分析揭示了独特的蛋白质，并为深入描述引起巴西CDI的菌株提供了额外的信息。(C)2017爱思唯尔有限公司。保留所有权利。
Clostridium difficile is a spore-forming anaerobic intestinal pathogen that causes Clostridium difficile infection (CDI). C. difficile is the leading cause of toxin-mediated nosocomial antibiotic-associated diarrhea. The pathogenesis of CDI is attributed to two major virulence factors, TcdA and TcdB toxins, that cause the symptomatic infection. C. difficile also expresses a number of key proteins, including cell wall proteins (CWPs). S-layer proteins (SLPs) are CWPs that form a paracrystalline surface array that coats the surface of the bacterium. SLPs have a role in C. difficile binding to the gastrointestinal tract, but their importance in virulence need to be better elucidated. Here, we describe bottom-up proteomics analysis of surface-enriched proteins fractions obtained through glycine extraction of five C. difficile clinical isolates from Brazil using gel-based and gel-free approaches. We were able to identify approximately 250 proteins for each strain, among them S1pA, Cwp2, Cwp6, CwpV and Cwp84. Identified CWPs presented different amino acid coverage, which might suggest differences in post-translational modifications. Proteomic analysis of SLPs from ribotype 133, agent of C. difficile outbreaks in Brazil, revealed unique proteins and provided additional information towards in depth characterization of the strains causing CDI in Brazil. (C) 2017 Elsevier Ltd. All rights reserved.