Recovery of Transgenic Plants by Agrobacterium-mediated Genetic Transformation in Dianthus caryophyllus L. (carnation)
Recovery of Transgenic Plants by Agrobacterium-mediated Genetic Transformation in Dianthus caryophyllus L. (carnation)
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发表时间：
2011
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通讯作者：
J. K. Kanwar;Surinder Kumar
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作者：
J. K. Kanwar;Surinder Kumar
石竹 L. cv. “Tempo”是世界上重要的切花。我们使用叶子作为遗传转化的靶组织。通过根癌农杆菌介导的转化，比较了两种具有抗虫性（cry 1AB）基因的再生系统的效率。选择100mg/l卡那霉素进行转化。转化频率受预处理和共培养时间的影响很大。利用愈伤组织再生系统获得最高转化率17.67%。通过PCR和昆虫毒性生物测定证实了外源基因的存在和表达。所选转化体在体外和体内条件下均显示出正常表型。
Dianthus caryophyllus L. cv. `Tempo’ is an important cut flower in the world. We used leaves as target tissue for genetic transformation. The efficiency of two regeneration systems with insect resistance (cry 1AB) gene was compared, through Agrobacterium tumefaciens mediated transformation. 100 mg/l kanamycin was selected for transformation. The transformation frequency was greatly affected by pre-conditioning and co-cultivation duration. The highest transformation frequency17.67% was obtained using callus regeneration system. The presence and expression of the foreign gene was confirmed by PCR and insect toxicity bioassay. The selected transformants showed normal phenotypes under in vitro and in vivo conditions.