Electron Microscopic Studies of Three Gliding Mycoplasmas, Mycoplasma mobile, M. pneumoniae, and M. gallisepticum, by Using the Freeze-Substitution Technique
Electron Microscopic Studies of Three Gliding Mycoplasmas, Mycoplasma mobile, M. pneumoniae, and M. gallisepticum, by Using the Freeze-Substitution Technique
复制标题
使用冷冻替代技术对三种滑动支原体（移动支原体、肺炎支原体和鸡毒支原体）进行电子显微镜研究
DOI：
10.1007/s00284-001-0014-8
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发表时间：
2002
影响因子：
2.6
通讯作者：
M. Miyata
中科院分区：
文献类型：
--
作者：
Takashi Shimizu;M. Miyata
摘要将冷冻置换技术应用于移动的支原体，M. pneumoniae和M.鸡败血症病毒，所有这些病毒都可以沿着锥形细胞末端的方向滑动。M.移动的呈现烧瓶样细胞形态。在锥形端周围发现了额外的层。M. pneumoniae的细胞表现出突出的膜延伸，即附着细胞器，由细胞内的低密度空间组成，并具有锚定至末端的丝状致密核心。与常规化学固定法相比，其细节结构更为明显。M.与在化学固定薄切片的图像中可以观察到的规则颗粒相比，鸡毒败血症病毒在细胞的后部呈现不规则的致密颗粒。
Abstract Freeze-substitution technique was applied to thin-sectioning electron microscopy of Mycoplasma mobile, M. pneumoniae, and M. gallisepticum, all of which can glide in the direction of the tapered cell end. M. mobile presented a flask-like cell morphology. An additional layer was found around the tapered end. The cell images of M. pneumoniae showed a protruding membrane extension, the attachment organelle, composed of a low density space inside the cells and featuring a filamentous dense core anchored to the terminal end. The detailed structures were more obvious than those observed by the conventional chemical fixation. The cells of M. gallisepticum presented irregular dense granules, in contrast to regular particles, which can be observed in the images of chemically fixed thin sections, in the rear portion of the cells.