Characteristic Pattern of Aroma Ester Formation from Banana, Melon, and Strawberry with Reference to the Substrate Specificity of Ester Synthetase and Alcohol Contents in Pulp
Characteristic Pattern of Aroma Ester Formation from Banana, Melon, and Strawberry with Reference to the Substrate Specificity of Ester Synthetase and Alcohol Contents in Pulp
复制标题
香蕉、甜瓜和草莓香气酯形成的特征模式与酯合成酶底物特异性和纸浆中酒精含量的关系
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
K. Chachin
中科院分区：
文献类型：
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作者：
Y. Ueda;A. Tsuda;J. Bai;N. Fujishita;K. Chachin
采用顶空气相色谱法对香蕉、甜瓜和草莓果实中的挥发性酯类物质进行了研究。在香蕉的情况下，发现乙酯和丁酯是主要的酯，异丁酯和异戊酯其次。甜瓜中以乙酯类为主，草莓中以甲酯类为主。在所有的情况下，这些水果，乙酸酯和丁酸酯是主要的酯。从这些果实中提取醇酰基CoA转移酶（酯合成酶）。当使用丙醇、异丁醇、丁醇和异戊醇作为底物时，观察到该酶的高反应性，而当使用甲醇、乙醇和戊醇时，观察到低反应性。香蕉和甜瓜中的酶与乙酰辅酶A、丙酰辅酶A和丁酰辅酶A反应较好，草莓中的酶除与上述3种酰基辅酶A反应较好外，还与戊酰辅酶A反应较好。支链酰基辅酶A（异丁酰基和异戊酰基）是酶的不良底物。在香蕉和甜瓜果肉中观察到的醇类中，乙醇占主导地位，而在草莓中则以甲醇为主。从这些观察，可以得出结论，从水果产生的酯具有反映果肉中的醇含量的醇部分，并且具有反映酯合成酶的酰基CoA特异性的酸部分。
Volatile esters produced from banana, melon and straw berry fruits were investigated by analyzing head-space gas. In the case of banana, ethyl and butyl esters were found to be main esters and isobutyl and isoamyl esters followed. In melon, ethyl esters were predominant, and in strawberry methyl esters were mainly observed. In all cases of these fruits, acetate and butyrate were the main esters. Alcohol acyl CoA transferase (ester synthetase) was extracted from these fruits. A high reactivity of the enzyme was observed when propyl, isobutyl, butyl and isoamyl alcohols were used as the substrates, whereas a low reactivity was observed when methyl, ethyl and amyl alcohols were used. The enzyme from banana and melon reacted well with acetyl CoA, propionyl CoA and butyryl CoA, while the enzyme from strawberry reacted well with valeryl CoA besides the above three acyl CoAs. Branched chain acyl CoAs (isobutyryl and isovaleryl) were poor substrates for the enzyme. Ethyl alcohol was predominant among alcohols observed in pulp of banana and melon fruits, whereas methyl alcohol was predominant in strawberry. From these observations, it may be concluded that esters produced from fruits have alcohol moieties reflected alcohol contents in the pulps and have acid moieties reflected acyl CoA specificity of the ester synthetase.