Decreased mineral content in MMP-20 null mouse enamel is prominent during the maturation stage
Decreased mineral content in MMP-20 null mouse enamel is prominent during the maturation stage
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DOI：
10.1177/154405910408301204
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发表时间：
2004-12-01
影响因子：
7.6
通讯作者：
Smith, CE
中科院分区：
文献类型：
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作者：
Bartlett, JD;Beniash, E;Smith, CE
在釉质发育期间，基质金属蛋白酶-20（MMP-20，釉质溶解素）在分泌阶段早期随着釉质增厚而表达，并且激肽释放酶-4（KLK-4，EMSP 1）在成熟阶段后期随着釉质硬化而表达。因此，我们研究了分泌/成熟阶段MMP-20无效釉质的物理性质是否与对照组有显著差异。我们证明，尽管相对而言，MMP-20缺失小鼠牙釉质中成熟矿物质的重量百分比仅比对照组低7-16%，但总体上牙釉质矿物质减少了约50%，其硬度降低了37%。在3个不同的发育阶段评估按重量计的矿物质含量百分比。值得注意的是，当MMP-20不再正常表达时，MMP-20无效和对照之间矿物质含量的最大差异发生在接近成熟的釉质中。这表明MMP-20直接或间接地促进成熟阶段釉质蛋白的去除。
During enamel development, matrix metalloproteinase-20 (MMP-20, enamelysin) is expressed early during the secretory stage as the enamel thickens, and kallikrein-4 (KLK-4, EMSP1) is expressed later during the maturation stage as the enamel hardens. Thus, we investigated whether the physical properties of the secretory/ maturation-stage MMP-20 null enamel were significantly different from those of controls. We demonstrated that although, in relative terms, the weight percent of mature mineral in the MMP-20 null mouse enamel was only 7-16% less than that in controls, overall the enamel mineral was reduced by about 50%, and its hardness was decreased by 37%. Percent mineral content by weight was assessed at 3 different developmental stages. Remarkably, the biggest difference in mineral content between MMP-20 null and controls occurred in the nearly mature enamel, when MMP-20 is normally no longer expressed. This suggests that MMP-20 acts either directly or indirectly to facilitate the removal of maturation-stage enamel proteins.