An Enzyme-Linked Immunosorbent Assay for Detection of Linear Alkylbenzene Sulfonate: Development and Field Studies
An Enzyme-Linked Immunosorbent Assay for Detection of Linear Alkylbenzene Sulfonate: Development and Field Studies
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用于检测直链烷基苯磺酸盐的酶联免疫吸附测定：开发和现场研究
DOI：
10.1021/es9707522
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Ken
中科院分区：
文献类型：
--
作者：
M. Fujita;M. Ike;Y. Goda;S. Fujimoto;Y. Toyoda;Ken
建立了一种精确的免疫分析系统，用于定量分析国内洗涤剂中应用最广泛的阴离子表面活性剂烷基苯磺酸盐（LAS）。为了制备las特异性单克隆抗体（mAb），将小鼠骨髓瘤细胞与经5-磺酸-5-戊酸偶联牛血清白蛋白免疫的小鼠脾细胞融合产生杂交瘤细胞。在使用竞争性酶联免疫吸附试验（ELISA）筛选后，选择了对LAS具有高结合亲和力的单抗，并用于开发敏感的竞争性直接ELISA。探测范围在20到500 ppb LAS之间。通过与传统的高效液相色谱（HPLC）和亚甲基蓝活性物质（MBAS）法分析河流样品中LAS和阴离子表面活性剂的方法进行比较，对ELISA法进行了优化和验证。ELISA法与HPLC法测定结果的相关系数与MB法测定结果的相关系数比较。
An accurate immunoassay system was developed for the quantitative analysis of linear alkylbenzene sulfonates (LAS), the most widely used anionic surfactants among domestic detergents. To generate LAS-specific monoclonal antibodies (mAb), hybridoma cells were produced by the fusion of mouse myeloma cells and spleen cells from mice immunized with sulfophenyl-5-valeric acid coupled to bovine serum albumin. After screening using a competitive enzyme-linked immunosorbent assay (ELISA), a mAb with a high binding affinity for LAS was selected and used in the development of a sensitive competitive direct ELISA. The detection range is between 20 and 500 ppb LAS. The ELISA assay was optimized and validated by comparison with conventional methods for the analysis of LAS and anionic surfactants, such as high-performance liquid chromatog raphy (HPLC) and methylene blue active substances (MBAS) methods, in river samples. The correlation coef ficients between the assay values obtained using the ELISA and the HPLC and MB...