Chromosome Numbers and Interspecific Hybrids Among New Guinea Impatiens Species
Chromosome Numbers and Interspecific Hybrids Among New Guinea Impatiens Species
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新几内亚凤仙花的染色体数目和种间杂种
DOI：
10.1093/oxfordjournals.jhered.a108358
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发表时间：
1973
影响因子：
3.1
通讯作者：
T. Arisumi
中科院分区：
文献类型：
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作者：
T. Arisumi
材料和方法植物作为生根插条从马里兰州Glenn Dale的植物引种站获得。它们将通过其植物介绍（PI）编号或登录号进行识别。将植物在自然光照下生长至成熟，并在夜间保持在约18摄氏度和白天保持在24摄氏度的遮蔽和遮蔽的温室中培育。由于所有供试品种均为先雄型，因此在开花期去雄，2 ~ 4天后柱头可接受时授粉。为了防止种子损失，用玻璃纸袋保护胶囊直到收获时，授粉后约30至35天。每个物种自交和与其他物种杂交，使用3至6次授粉。重复杂交和自花授粉，直到收获至少三个蒴果。在25至30次授粉后未能结籽的杂交或自交被中止。来自这些杂交和自交的种子发芽
Materials and MethodsThe plants were obtained as rooted cuttings from the Plant Introduction Station, Glenn Dale, Maryland. They will be identified by their Plant Introduction (PI) numbers or by accession numbers. The plants were grown to maturity under natural illumination, and bred in a screened and shaded greenhouse maintained at about 18 degrees C at night and 24 degrees C during the day. Because all the species under test were protandrous, the flowers were emasculated at anthesis and pollinated 2 to 4 days later when the stigma became receptive. To prevent loss of seeds, the capsules were protected with glassine bags until time of harvest, about 30 to 35 days after pollination. Each species was selfed and crossed with other species, using 3 to 6 pollinations. The crosses and self-pollinations were repeated until at least three capsules had been harvested. Crosses or selfs that failed to set seed after 25 to 30 pollinations were discontinued. The seeds from these crosses and selfs were germinated