Formation and composition of the peritrophic membrane in the sand fly, Phlebotomus perniciosus (Diptera: Psychodidae).
Formation and composition of the peritrophic membrane in the sand fly, Phlebotomus perniciosus (Diptera: Psychodidae).
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DOI：
10.1093/jmedent/30.1.179
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发表时间：
1993
影响因子：
2.1
通讯作者：
L. L. Walters-L.;K. P. Irons;H. Guzmán;R. Tesh
中科院分区：
文献类型：
--
作者：
L. L. Walters-L.;K. P. Irons;H. Guzmán;R. Tesh
对白蛉围食膜的分泌物、形态和化学成分进行了研究。该膜由整个中肠上皮分泌。电子致密的细颗粒状分泌物，可能是几丁质，目前沿着长度的微绒毛立即，直到24小时后喂养。从12-48小时，电子透明的粗颗粒成分，可能是蛋白质，也分泌的微绒毛表面。光镜下，成熟的36-h膜特征性地由深色的前帽和后开口环组成，透明的中间膜和前塞。在24-48小时完全形成的膜的超微结构是高度可变的。未分化的膜出现作为一个单一的电子透明层，分化膜更复杂，有时两层，包含电子透明和致密的纤维和颗粒。结合琥珀酰化麦胚凝集素，组织化学和氨基酸分析的结果表明，膜由几丁质，糖蛋白和蛋白质。在膜蛋白中鉴定了18种氨基酸;天冬氨酸-谷氨酸（和酰胺）、丝氨酸、甘氨酸和赖氨酸（45%重量）在交联膜组分中可能是重要的。
The secretion, morphology, and chemical composition of the peritrophic membrane was studied in the sand fly, Phlebotomus perniciosus Newstead. The membrane was secreted from the entire midgut epithelium. An electron-dense fine granular secretion, possibly chitin, was present along the length of the microvilli immediately until 24 h after feeding. From 12-48 h, an electron-lucid coarse granular component, possibly protein, was also secreted from the microvillar surface. By light microscopy, the mature 36-h membrane characteristically consisted of a dark anterior cap and posterior open ring, with a transparent intervening membrane and anterior plug. Ultrastructure of the fully formed membrane at 24-48 h was highly variable. Undifferentiated membranes appeared as a single electron-lucid layer; differentiated membranes were more complex, sometimes two-layered, containing electron-lucid and -dense fibers and granules. Results of binding to succinylated wheat germ agglutinin, histochemistry, and amino acid analysis indicated that the membrane was composed of chitin, glycoprotein, and protein. Eighteen amino acids were identified in membrane proteins; aspartic-glutamic acids (and amides), serine, glycine, and lysine (45% by weight) may be important in cross-linking membrane components.