Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes.
Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes.
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DOI：
10.1007/978-1-4939-8570-8_3
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jakubzick CV
中科院分区：
文献类型：
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作者：
Gibbings SL;Jakubzick CV
在肺中存在不同的单核吞噬细胞（MP）群体，其由巨噬细胞、树突状细胞（DC）和单核细胞组成。这些不同细胞类型的存在表明，在稳态和炎症条件下，MP之间存在明确的劳动分工和微妙的平衡。在这里，我们描述了如何使用流式细胞术来识别肺MP，以及如何通过细胞分选来分离它们。在稳态条件下，鼠肺含有均匀的肺泡巨噬细胞（AM）群体，三种不同的间质巨噬细胞（IM）群体，三种DC亚型和少量的组织运输单核细胞。在炎症反应期间，单核细胞群体更丰富和复杂，因为它获得巨噬细胞样或DC样特征。总而言之，研究这些细胞类型如何相互作用，结构细胞和环境中的其他白细胞对于理解它们在维持稳态和疾病发展过程中的作用将是重要的。
There is a diverse population of mononuclear phagocytes (MPs) in the lungs, comprised of macrophages, dendritic cells (DCs), and monocytes. The existence of these various cell types suggests that there is a clear division of labor and delicate balance between the MPs under steady-state and inflammatory conditions. Here we describe how to identify pulmonary MPs using flow cytometry and how to isolate them via cell sorting. In steady-state conditions, murine lungs contain a uniform population of alveolar macrophages (AMs), three distinct interstitial macrophage (IM) populations, three DC subtypes, and a small number of tissue-trafficking monocytes. During an inflammatory response, the monocyte population is more abundant and complex since it acquires either macrophage-like or DC-like features. All in all, studying how these cell types interact with each other, structural cells, and other leukocytes within the environment will be important to understanding their role in maintaining homeostasis and during the development of disease.