CHARACTERIZATION OF MYELIN PROTEOLIPID MESSENGER-RNAS IN NORMAL AND JIMPY MICE
CHARACTERIZATION OF MYELIN PROTEOLIPID MESSENGER-RNAS IN NORMAL AND JIMPY MICE
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DOI：
10.1128/mcb.6.11.3755
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发表时间：
1986-11-01
影响因子：
5.3
通讯作者：
DEININGER, PL
中科院分区：
文献类型：
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作者：
GARDINIER, MV;MACKLIN, WB;DEININGER, PL
从17日龄大鼠脑干mRNA的pUC 18中制备的cDNA文库中分离出对大鼠髓鞘蛋白脂质蛋白（PLP）特异的克隆。该克隆对应于PLP编码区的羧基末端的第三个。该克隆用于鉴定小鼠脑中PLP特异性mRNA，并建立小鼠脑发育期间PLP mRNA表达的时间过程。三种PLP特异性mRNA被观察到，长度约为1，500，2，400和3，200个碱基，其中最大的是最丰富的。在脑发育过程中，PLP mRNA表达的高峰期为14 ~ 25日龄，这与髓鞘碱性蛋白mRNA表达的时间进程相似。当jimpy小鼠，一个X-连锁髓鞘形成障碍的突变体，PLP mRNA的表达进行了研究，低水平的PLP mRNA被认为是野生型水平的约5%，在20天的年龄。当通过北方印迹分析jimpy脑RNA时，PLP特异性mRNA显示比野生型PLP特异性mRNA短100至200个碱基。这种大小差异见于两种主要的PLP mRNA中，并且它不是由这些mRNA的多聚腺苷酸化的损失引起的。
A clone specific for the rat myelin proteolipid protein (PLP) was isolated from a cDNA library made in pUC18 from 17-day-old rat brain stem mRNA. This clone corresponded to the carboxyl-terminal third of the PLP-coding region. The clone was used to identify PLP-specific mRNAs in mouse brain and to establish the time course of PLP mRNA expression during mouse brain development. Three PLP-specific mRNAs were seen, approximately 1,500, 2,400, and 3,200 bases in length, of which the largest was the most abundant. During brain development, the maximal period of PLP mRNA expression was from 14 to 25 days of age, and this was a similar time course to that for myelin basic protein mRNA expression. When the jimpy mouse, an X-linked dysmyelination mutant, was studied for PLP mRNA expression, low levels of PLP mRNA were seen which were approximately 5% of wild-type levels at 20 days of age. When jimpy brain RNA was analyzed by Northern blotting, the PLP-specific mRNA was shown to be 100 to 200 bases shorter than the wild-type PLP-specific mRNA. This size difference was seen in the two major PLP mRNAs, and it did not result from a loss of polyadenylation of these mRNAs.