Analysis of modified oligonucleotides by capillary electrophoresis in a polyvinylpyrrolidone matrix coupled with electrospray mass spectrometry
Analysis of modified oligonucleotides by capillary electrophoresis in a polyvinylpyrrolidone matrix coupled with electrospray mass spectrometry
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DOI：
10.1016/0021-9673(95)01239-7
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发表时间：
1996-04-26
影响因子：
4.1
通讯作者：
Vouros, P
中科院分区：
文献类型：
--
作者：
Barry, JP;Muth, J;Vouros, P
This paper describes the coupling of capillary electrophoresis (CE) with a polyvinylpyrrolidone (PVP) matrix and electrospray mass spectrometry for the analysis of a series of short modified oligonucleotides. The separation of modified oligonucleotides, four, five and six bases in length, was accomplished by CE in a coated fused-silica column filled with an aqueous solution of PVP. The resolved components were then identified by electrospray mass spectrometry with negative-ion detection. Importantly, oligonucleotides with the same length and sequence but differing only by the presence or absence of a small modification (such as a methyl group) on a single base were easily resolved by CE. This observation suggests that the matrix, PVP, is behaving as a pseudo-phase in which oligomers with hydrophobic modifications are retained longer than their normal unmodified analogs.
