Comparison of microCT and an inverse finite element approach for biomechanical analysis: results in a mesenchymal stem cell therapeutic system for fracture healing.
Comparison of microCT and an inverse finite element approach for biomechanical analysis: results in a mesenchymal stem cell therapeutic system for fracture healing.
复制标题
用于生物力学分析的 microCT 和逆有限元方法的比较：产生用于骨折愈合的间充质干细胞治疗系统。
DOI：
10.1016/j.jbiomech.2012.05.033
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发表时间：
2012
影响因子：
2.4
通讯作者：
Miga,MichaelI
中科院分区：
文献类型：
--
作者：
Weis,JaredA;Granero-Moltó,Froilán;Myers,TimothyJ;Longobardi,Lara;Spagnoli,Anna;Miga,MichaelI
骨折愈合研究中的一个重要问题是评估小动物系统中候选治疗方法的机械完整性的能力。在最近的报道中，有人提出微ct图像衍生的密度测量参数可以用作力学性能评估的替代品。最近，我们提出了一种逆方法，通过在逆有限元分析（FEA）中整合内在力学特性（来自生物力学测试）和几何信息（来自微ct），迭代重建集总软/硬愈伤组织区域的弹性模量，以定义愈伤组织质量测量。在本文中，从涉及间充质干细胞的治疗系统的数据是在比较传统的微ct密度测量和力学性能指标的背景下进行分析。此外，还分析了一种新的多参数回归微ct参数以及我们的反有限元度量。结果表明，反向有限元分析方法是成功检测纵向和治疗反应的唯一指标。虽然最有希望的基于微ct的指标在早期愈合状态下是足够的，但它们无法追踪晚期的机械完整性。此外，我们的分析通过证明MSCs加速愈合的作用增加了洞察力，并且是证明晚期愈合治疗益处的唯一指标。总之，本文提出的工作表明，微ct密度参数是机械完整性的不完全替代。此外，我们的反向有限元分析方法被证明是非常敏感的，可能为规范评估愈合骨折机械完整性的通常具有挑战性的过程提供了第一步。
An important concern in the study of fracture healing is the ability to assess mechanical integrity in response to candidate therapeutics in small-animal systems. In recent reports, it has been proposed that microCT image-derived densitometric parameters could be used as a surrogate for mechanical property assessment. Recently, we have proposed an inverse methodology that iteratively reconstructs the modulus of elasticity of the lumped soft callus/hard callus region by integrating both intrinsic mechanical property (from biomechanical testing) and geometrical information (from microCT) within an inverse finite element analysis (FEA) to define a callus quality measure. In this paper, data from a therapeutic system involving mesenchymal stem cells is analyzed within the context of comparing traditional microCT densitometric and mechanical property metrics. In addition, a novel multi-parameter regression microCT parameter is analyzed as well as our inverse FEA metric. The results demonstrate that the inverse FEA approach was the only metric to successfully detect both longitudinal and therapeutic responses. While the most promising microCT-based metrics were adequate at early healing states, they failed to track late-stage mechanical integrity. In addition, our analysis added insight to the role of MSCs by demonstrating accelerated healing and was the only metric to demonstrate therapeutic benefits at late-stage healing. In conclusion, the work presented here indicates that microCT densitometric parameters are an incomplete surrogate for mechanical integrity. Additionally, our inverse FEA approach is shown to be very sensitive and may provide a first-step towards normalizing the often challenging process of assessing mechanical integrity of healing fractures.
影响因子：
3.1
作者：
Sullivan, JM;Charron, G;Paulsen, KD
通讯作者：
Paulsen, KD
影响因子：
2.4
作者：
Weis, Jared A.;Miga, Michael I.;Granero-Molto, Froilan;Spagnoli, Anna
通讯作者：
Spagnoli, Anna