ENZYMES OF SALVAGE AND DENOVO PATHWAYS OF SYNTHESIS OF PYRIMIDINE NUCLEOTIDES IN HUMAN COLORECTAL ADENOCARCINOMAS
ENZYMES OF SALVAGE AND DENOVO PATHWAYS OF SYNTHESIS OF PYRIMIDINE NUCLEOTIDES IN HUMAN COLORECTAL ADENOCARCINOMAS
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DOI：
10.1016/0006-2952(82)90058-2
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发表时间：
1982-01-01
影响因子：
5.8
通讯作者：
WELCH, AD
中科院分区：
文献类型：
--
作者：
AHMED, NK;HAGGITT, RC;WELCH, AD
尿苷-胞苷激酶（Urd-Cyd 激酶）是修复嘧啶核苷的关键酶，其活性平均为 0.86 .+-。 0.16 nmol 磷酸尿苷.cntdot。 min-1 .cntdot。 (mg 蛋白质)-1 存在于 53 个人类结直肠腺癌标本中。 35个癌标本中氟尿嘧啶磷酸核糖基转移酶(FUPRTase)的活性平均仅为0.19.+-。 0.07 nmol 氟尿苷磷酸盐.cntdot。 min-1 .cntdot。 （毫克蛋白质）-1。 UMP生物合成从头途径中最后一种酶，即乳清苷5''-单磷酸(OMP)脱羧酶的活性，平均为0.21.+-。 0.04 nmol CO2 .cntdot。 min-1 .cntdot。 （毫克蛋白质）-1。 Urd-Cyd 激酶的活性增加约。 2.3 倍，以及 OMP 脱羧酶的 .apprx 倍。与正常人结肠粘膜相比，FUPRTase 的酶活性仅增加了 91%，而 FUPRTase 的酶活性仅增加了 27%。在所研究的结直肠癌中，72%为中分化，21%为低分化，7%为高分化。 53 个癌的平均直径为 5.5 cm，病理分期将 15% 分类为 Dukes'' A，36% 分类为 Dukes'' B，47% 为 Dukes'' C，2% 为原位癌。无法辨别所研究的酶活性水平与癌症的任何病理特征之间的相关性。
The activity of uridine-cytidine kinase (Urd-Cyd kinase) a key enzyme in the salvage of pyrimidine nucleosides, averaged 0.86 .+-. 0.16 nmol uridine phosphates .cntdot. min-1 .cntdot. (mg protein)-1 in 53 specimens of human colorectal adenocarcinoma. The activity of fluorouracil phosphoribosyltransferase (FUPRTase) in 35 carcinoma specimens averaged only 0.19 .+-. 0.07 nmol fluorouridine phosphates .cntdot. min-1 .cntdot. (mg protein)-1. The activity of the last enzyme in the de novo pathway of biosynthesis of UMP, i.e., orotidine 5''-monophosphate (OMP) decarboxylase, averaged 0.21 .+-. 0.04 nmol CO2 .cntdot. min-1 .cntdot. (mg protein)-1. The activity of Urd-Cyd kinase was increased .apprx. 2.3-fold, and that of OMP decarboxylase by .apprx. 91% while that of FUPRTase was increased by only 27%, as compared to that of normal human colonic mucosa. Of the colorectal carcinomas studied, 72% were moderately differentiated, 21% poorly differentiated and 7% well differentiated. The mean diameter of the 53 carcinomas was 5.5 cm, and pathologic staging led to classification of 15% as Dukes'' A, 36% as Dukes'' B, 47% as Dukes'' C and 2% as carcinoma in situ. No correlations between the level of the enzyme activities studied and any pathologic characteristics of the carcinomas could be discerned.