Positive effect of HPV status on prognostic value of blood lymphocyte-to-monocyte ratio in advanced cervical carcinoma.
Positive effect of HPV status on prognostic value of blood lymphocyte-to-monocyte ratio in advanced cervical carcinoma.
复制标题
HPV 状态对晚期宫颈癌血液淋巴细胞与单核细胞比率的预后价值有积极影响。
DOI：
10.1186/s12935-016-0334-1
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发表时间：
2016
影响因子：
5.8
通讯作者：
Xu LH
中科院分区：
文献类型：
--
作者：
Li SW;Yuan W;Zhao B;He ZK;Guo X;Xia WX;Xu LH
本研究旨在探讨预处理淋巴细胞/单核细胞比（LMR）对局部晚期宫颈癌预后的意义及其对总生存期的影响。常规血常规定量检测LMR。用相对光单位（RLU）与1 pg/mL的HPV 16型DNA阳性对照（RLU/PC）样品比较，分析了杂交捕获2检测到的宫颈癌样品中人乳头瘤病毒（HPV）型DNA的信号强度。以1.0 RLU/PC （~1 pg/mL）为阳性阈值。hpv阳性标本采用反杂交线探针法分型。LMR和HPV DNA分别是5年总生存期（OS）和无进展生存期的独立预后标志物。它们的联合检测可以进一步提高对OS的预测价值。在HR（高风险）-HPV DNA状态阳性亚组中，LMR对改善OS有积极作用，但在非HR HPV DNA状态亚组中则没有。LMR和HR-HPV DNA状态可被确定为独立的预后因素。放化疗中LMR对生存的不同影响可能与HR-HPV DNA状态有关。联合检测LMR和HR-HPV DNA状态可能有助于筛查预后。
This retrospective study aimed to investigate the prognostic significance of pretreatment lymphocyte-to-monocyte ratio (LMR) in locally advanced cervical cancer and its effect on overall survival. The usual blood routine test was quantitatively performed to detect LMR. Signal strengths of human papilloma virus (HPV) type DNA in detected cervical cancer samples using hybrid capture 2 were analyzed in relative light units (RLU) compared with 1 pg/mL of HPV type 16 DNA-positive control (RLU/PC) samples. A total of 1.0 RLU/PC (~1 pg/mL) was used as the threshold for a positive result. The HPV-positive specimens were typed using reverse-hybridization line probe assay. The LMR and HPV DNA were found to be independent prognostic markers for 5-year overall survival (OS) and progression-free survival, respectively. Their joint detection may further enhance the predictive value for OS. In the positive HR (high risk)-HPV DNA status subgroup, LMR had a positive effect on improved OS but not in the non-HR HPV DNA status subgroup. The LMR and HR-HPV DNA status can be identified as independent prognostic factors. The different influences of LMR in combined chemoradiotherapy on survival may be related to HR-HPV DNA status. The combined detection of LMR and HR-HPV DNA status may contribute to screening prognosis.