CONSTITUTIVE OVEREXPRESSION OF A GROWTH-REGULATED GENE IN TRANSFORMED CHINESE-HAMSTER AND HUMAN-CELLS
CONSTITUTIVE OVEREXPRESSION OF A GROWTH-REGULATED GENE IN TRANSFORMED CHINESE-HAMSTER AND HUMAN-CELLS
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DOI：
10.1073/pnas.84.20.7188
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发表时间：
1987-10-01
影响因子：
11.1
通讯作者：
SAGER, R
中科院分区：
文献类型：
--
作者：
ANISOWICZ, A;BARDWELL, L;SAGER, R
通过消减杂交的比较发现，在高致瘤性的CHEF/16细胞中存在中等丰富的信息，而在密切相关的非致瘤性CHEF/18细胞中存在非常低的水平。克隆和测序后，计算机比对显示与人结缔组织激活肽III(CTAP III)的同源性最高。与中国仓鼠克隆杂交的人肿瘤细胞的cDNA被分离、测序，发现与中国仓鼠的基因比与CTAP III更接近。因此，从中国仓鼠和人细胞中克隆的cDNAs代表一个不同的基因，命名为gro。对其转录调控的研究表明，在正常的中国仓鼠和人类细胞中，它的表达受到生长状态的严格调控，而在迄今所研究的致瘤细胞中，它的表达受到放松。
Comparison by subtractive hybridization of mRNAs revealed a moderately abundant message in highly tumorigenic CHEF/16 cells present at very low levels in closely related nontumorigenic CHEF/18 cells. After cloning and sequencing the corresponding cDNA, computer comparison showed closest homology with the human connective tissue-activating peptide III (CTAP III). The human tumor cell cDNA hybridizing with the Chinese hamster clone was isolated, sequenced, and found to have closer similarity to the Chinese hamster gene than to CTAP III. Thus, the cloned cDNAs from Chinese hamster and human cells represent a different gene, named gro. Studies of its transcriptional regulation have shown that expression is tightly regulated by growth status in normal Chinese hamster and human cells and relaxed in the tumorigenic cells so far examined.