High STMN1 Expression is Associated with Cancer Progression and Chemo-Resistance in Lung Squamous Cell Carcinoma
High STMN1 Expression is Associated with Cancer Progression and Chemo-Resistance in Lung Squamous Cell Carcinoma
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DOI：
10.1245/s10434-017-6083-0
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发表时间：
2017-12-01
影响因子：
3.7
通讯作者：
Kuwano, Hiroyuki
中科院分区：
文献类型：
--
作者：
Bao, Pinjie;Yokobori, Takehiko;Kuwano, Hiroyuki
STMN1（基因符号：STMN1）被称为微管不稳定蛋白，调节微管的动力学、细胞周期进程和对紫杉烷类药物的化学抗性。应用免疫组织化学方法检测186例肺鳞癌（LSCC）患者的FFPE组织切片中STMN 1的表达。通过对STMN1小干扰RNA（siRNA）转染的LSCC细胞系进行STMN1抑制分析，观察其增殖、侵袭和凋亡能力以及对紫杉醇敏感性的变化。高表达与肿瘤血管浸润及预后有关（P = 0.0477）。此外，STMN 1抑制的LSCC细胞的增殖和侵袭能力均较对照组降低，而凋亡能力和紫杉醇敏感性均较对照组增加，提示STMN 1是一个与肿瘤进展和化疗耐药相关的预后因子。因此，STMN 1可能是喉鳞状细胞癌预后不良的预测因子和潜在的治疗靶点。
Known as a microtubule-destabilizing protein, STMN1 (gene symbol: STMN1) regulates the dynamics of microtubules, cell cycle progress, and chemo-resistance against taxane agents. It is highly expressed in various human cancers and involved in cancer progression as well as poor prognosis.Expression of STMN1 was examined by immunohistochemistry using FFPE tissue sections from 186 patients with lung squamous cell carcinoma (LSCC). Analysis of STMN1 suppression was performed for STMN1 small interfering RNA (siRNA)-transfected LSCC cell lines to determine the change in proliferation, invasive and apoptosis abilities, and paclitaxel sensitivity.The cytoplasmic STMN1 expression in LSCC was higher than in normal tissues. The high expression was significantly associated with vascular invasion (P = 0.0477) and poor prognosis. In addition, the proliferating and invasive abilities were decreased, and the apoptosis ability and paclitaxel sensitivity were increased in STMN1-suppressed LSCC cells compared with control cells.The results suggest that STMN1 is a prognostic factor that also is associated with caner progression and chemo-resistance. Therefore, STMN1 could be a predictor for poor prognosis and a potential therapeutic target in LSCC.