Effects of Artificial Extracellular Matrixes on the Survival of Vascular Endothelial Cells
Effects of Artificial Extracellular Matrixes on the Survival of Vascular Endothelial Cells
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人工细胞外基质对血管内皮细胞存活的影响
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Yang Jun
中科院分区：
文献类型：
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作者：
Yu Mei-Hua;Du Feng-Yi;Rao Xia;Yao Fang-Lian;Yang Jun
为了研究细胞外基质材料对内皮细胞的影响，将胶原蛋白、多聚赖氨酸和融合蛋白VEGF-Fc分别以物理吸附的方式固定在聚苯乙烯细胞培养板上。这三种蛋白显著改善了聚苯乙烯表面的亲水性。同时，研究了细胞的黏附、增殖以及细胞骨架蛋白和von Willebrand因子(VWF)的表达。结果表明，这些基质材料有效地促进了人脐静脉内皮细胞的黏附。在没有额外VEGF的情况下，在融合蛋白VEGF-Fc基质上培养的人脐静脉内皮细胞也可以保持和延长细胞的增殖和vWF的表达在胶原和融合蛋白基质上培养的人脐静脉内皮细胞表达丰富的应力纤维和丝状结构，因此，本工作构建的胶原和融合蛋白VEGF-Fc基质为人脐静脉内皮细胞提供了适宜的培养环境，为实现植入材料的内皮化和促进血管生成提供了理论依据。
In order to study the impact of extracellular matrix material on endothelial cells,collagen,poly-lysine and fusion protein VEGF-Fc were immobilized on the polystyrene cell culture plate by physical adsorbing,respectively.The three proteins significantly improved the hydrophilic of the polystyrene surface.Meanwhile,the cell adhesion,proliferation and the expressions of the cytoskeletal protein and von Willebrand factor(vWF) were investigated.The results showed that these matrixes effectively improved the adhesion of Human umbilical vein endothelial cells(HUVECs).And HUVECs cultured on fusion protein VEGF-Fc matrix without extra VEGF in the culture medium could also maintain and prolong the bioactivities of cell proliferation and vWF expression.Furthermore,HUVECs cultured on collagen and fusion protein matrixes expressed abundant stress fibers and filopodia.Thereby,the collagen and fusion protein VEGF-Fc matrixes constructed in this work provided suitable environment for HUVECs.Those results will provide a theoretical basis on achieving the endothelialization and improving the angiogenesis of implanted materials.