GmSBH1, a homeobox transcription factor gene, relates to growth and development and involves in response to high temperature and humidity stress in soybean
GmSBH1, a homeobox transcription factor gene, relates to growth and development and involves in response to high temperature and humidity stress in soybean
复制标题
GmSBH1 是同源盒转录因子基因，与大豆生长发育相关，并参与对高温和潮湿胁迫的响应
DOI：
10.1007/s00299-015-1840-7
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发表时间：
2015-11-01
影响因子：
6.2
通讯作者：
Ma, Hao
中科院分区：
文献类型：
--
作者：
Shu, Yingjie;Tao, Yuan;Ma, Hao
GmSBH1参与植物对高温高湿胁迫的应答，同源异型盒转录因子是控制植物发育过程的关键开关。Glycine max H1 Sbh1（GmSBH1）是第一个从大豆中分离到的同源异型盒基因。在本研究中，GmSBH1的完整的ORF被分离，并发现编码的蛋白质是一个典型的I类KNOX同源框转录因子。亚细胞定位和转录激活实验表明，GmSBH 1是一个核蛋白，并具有转录激活活性的同源结构域。发现KNOX 1结构域在抑制GmSBH 1的转录激活活性中起明确的作用。GmSBH1基因在大豆不同组织中的表达量不同，并参与大豆种子发育过程中对高温高湿胁迫的响应。GmSBH1在拟南芥中的过表达改变了叶片和气孔表型，并增强了种子对HTH胁迫的耐受性。总之，我们的研究结果表明，GmSBH1参与大豆的生长，发育，并提高对HTH胁迫引起的收获前种子劣变的耐受性。
GmSBH1 involves in response to high temperature and humidity stress.Homeobox transcription factors are key switches that control plant development processes. Glycine max H1 Sbh1 (GmSBH1) was the first homeobox gene isolated from soybean. In the present study, the full ORF of GmSBH1 was isolated, and the encoded protein was found to be a typical class I KNOX homeobox transcription factor. Subcellular localization and transcriptional activation assays showed that GmSBH1 is a nuclear protein and possesses transcriptional activation activity in the homeodomain. The KNOX1 domain was found to play a clear role in suppressing the transcriptional activation activity of GmSBH1. GmSBH1 showed different expression levels among different soybean tissues and was involved in response to high temperature and humidity (HTH) stress in developing soybean seeds. The overexpression of GmSBH1 in Arabidopsis altered leaf and stoma phenotypes and enhanced seed tolerance to HTH stress. Overall, our results indicated that GmSBH1 is involved in growth, development, and enhances tolerance to pre-harvest seed deterioration caused by HTH stress in soybean.