RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT
RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT
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DOI：
10.18999/nagjms.76.3-4.293
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发表时间：
2014-08
影响因子：
0.9
通讯作者：
Mayuko Kishimoto;Takenori Matsuda;Shougo Yanase;A. Katsumi;N. Suzuki;M. Ikejiri;A. Takagi;M. Ikawa;T. Kojima;S. Kunishima;H. Kiyoi;T. Naoe;T. Matsushita;M. Maruyama
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文献类型：
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作者：
Mayuko Kishimoto;Takenori Matsuda;Shougo Yanase;A. Katsumi;N. Suzuki;M. Ikejiri;A. Takagi;M. Ikawa;T. Kojima;S. Kunishima;H. Kiyoi;T. Naoe;T. Matsushita;M. Maruyama
摘要RhoF是Rho GTP酶家族中的一员，参与多种细胞功能，包括细胞的长丝足形成、黏附和迁移。虽然RhoF在淋巴组织中表达，但RhoF在B细胞发育中的作用仍很不清楚。另一方面，Rho GTPase家族的其他成员，如CDC42、RhoA和Rac，已经被深入研究，并被认为是骨髓和脾中B细胞发育所必需的。我们假设RhoF也参与B细胞的发育。为了验证我们的假设，我们分析了RhoF基因敲除(KO)小鼠的B细胞发育，发现脾中的边缘带(MZ)B细胞显著减少，尽管胸腺和脾中的T细胞发育没有受到影响。与这些结果一致的是，RhoF KO小鼠脾中MZ B细胞区的宽度显著减少。而MZ B细胞特异性抗原(T细胞非依赖性抗原，I型)刺激后的IgM和IgG3抗体滴度不受RhoF缺失的影响。此外，我们还证明，在基质细胞衍生因子-1α-和B淋巴细胞趋化因子诱导的B细胞迁移过程中，ROF是必不可少的。这些结果表明，RhoF促进了脾中MZ B细胞的发育。
ABSTRACT RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation, adhesion, and migration of cells. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. On the other hand, other members of the Rho GTPase family, such as Cdc42, RhoA, and Rac, have been intensively studied and are known to be required for B cell development in the bone marrow and spleen. We hypothesized that RhoF is also involved in B cell development. To examine our hypothesis, we analyzed B cell development in RhoF knockout (KO) mice and found a significant reduction in marginal zone (MZ) B cells in the spleen, although T cell development in the thymus and spleen was not affected. Consistent with these results, the width of the MZ B cell region in the spleen was significantly reduced in the RhoF KO mice. However, the antigen-specific antibody titer of IgM and IgG3 after MZ B cell-specific antigen (T cell-independent antigen, type I) stimulation was not affected by RhoF deletion. Furthermore, we demonstrated that RhoF was dispensable for stromal cell-derived factor-1α- and B lymphocyte chemoattractant-induced B cell migration. These results suggest that RhoF promotes MZ B cell development in the spleen.