Experimental study of ultrasound retention of bubble-surrounded cells under various conditions of acoustic field and flow velocity
Experimental study of ultrasound retention of bubble-surrounded cells under various conditions of acoustic field and flow velocity
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DOI：
10.35848/1347-4065/ac54f9
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发表时间：
2022-07-01
影响因子：
1.5
通讯作者：
Masuda, Kohji
中科院分区：
文献类型：
--
作者：
Chikaarashi, Takumi;Watanabe, Shunya;Masuda, Kohji
我们证实，气泡包围细胞（BSCs）中包含的流动保留在人造血管的壁上，通过形成一个声场与多个焦点，使用时空分割发射。为了实现细胞递送系统，我们研究了显微图像中T细胞浓度与亮度之间的关系。接下来，我们定义了应用的声强度，从每种类型的声场的声压分布导出。我们研究了不同流速、焦点数目和空间间隔以及最大声压对BSC保留性能的影响。从结果来看，保留细胞的最佳声场取决于声强度和流速，其中在10-20 mm s（-1）的流速范围内，声强度为50-120 mW cm（-2）的多个焦点比声强度为180 mW cm（-2）的单个焦点更有效。
We confirmed that bubble-surrounded cells (BSCs) contained in flow were retained on the walls of an artificial blood vessel by forming an acoustic field with multiple focal points using tempo-spatial division emission. In order to realize the cell delivery system, we investigated the relationship between the concentration of T-cells and brightness in the microscopic images. Next, we defined the applied acoustic intensity, derived from the sound pressure distribution of every type of acoustic field. We studied the retention performance of BSCs versus various flow velocities, number and spatial intervals of the focal points, and maximum sound pressure. From the results, the optimal acoustic field to retain the cells depends on both acoustic intensity and flow velocity, where multiple focal points with an acoustic intensity of 50-120 mW cm(-2) were more effective than the single focal point with 180 mW cm(-2) in the range of a flow velocity of 10-20 mm s(-1).