Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells In-vitro
Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells In-vitro
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DOI：
10.22037/ijpr.2015.1624
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Yong Peng;Z. Fu;Cong-Shan Guo;Yanxia Zhang;Ya Di;Bin Jiang;Qing-wang Li
中科院分区：
文献类型：
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作者：
Yong Peng;Z. Fu;Cong-Shan Guo;Yanxia Zhang;Ya Di;Bin Jiang;Qing-wang Li
本研究旨在观察黄芩苷对人宫颈癌HeLa细胞的诱导凋亡作用及其机制。采用MTT法测定黄芩苷对HeLa细胞生长的抑制作用，采用细胞划痕实验分析细胞增殖和迁移能力。光镜和电镜观察凋亡细胞的形态变化，流式细胞术确认细胞生长停滞。此外，Western blot用于研究凋亡相关蛋白的表达；使用分光光度法检查 Caspase-3 的激活。结果表明，黄芩苷可通过诱导细胞凋亡来抑制HeLa细胞的增殖，且呈时间和剂量依赖性（P<0.01）。黄芩苷诱导的细胞凋亡信号的特点是上调Bax、Fas、FasL和Caspase-8蛋白表达，并下调Bcl-2蛋白表达。这些结果表明，黄芩苷诱导的细胞凋亡涉及通过细胞内线粒体途径和表面死亡受体途径激活HeLa细胞中的Caspase-3。
The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscope, and cell growth arrest was confirmed by flow cytometry. Moreover, Western blot was used for investigating the expression of apoptosis related proteins; spectrophotometry was used to examine Caspase-3 activation. Our results showed that baicalin could inhibit the proliferation of HeLa Cells via induction of apoptosis in a time and dose-dependent manner (P<0.01). Apoptotic signaling induced by baicalin was characterized by up-regulating Bax, Fas, FasL and Caspase-8 protein expression, and down-regulating of Bcl-2 protein expression. These results indicated that baicalin-induced apoptosis involved activation Caspase-3 in HeLa cells through the intracellular mitochondrial pathway and the surface death receptor pathway.