A global view of gene expression in the preimplantation mouse embryo: morula versus blastocyst
A global view of gene expression in the preimplantation mouse embryo: morula versus blastocyst
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DOI：
10.1016/j.ejogrb.2004.01.026
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发表时间：
2004-07-01
影响因子：
2.6
通讯作者：
Ko, MSH
中科院分区：
文献类型：
--
作者：
Tanaka, TS;Ko, MSH
作为了解植入前发育的第一步，我们使用NIA 15 k小鼠cDNA微阵列对桑椹胚和囊胚进行了全球基因表达谱分析。对胚泡和桑椹胚分别测量四次和五次基因表达水平。在5%显著性水平下的学生t检验鉴定出与桑椹胚相比，胚泡中428个基因上调，748个基因下调。这种趋势与样品基因的半定量RT-PCR分析一致。已知参与关键调控过程的上调基因包括Mist 1、Id 2、Hd 1和miwi 1;下调基因包括CREB结合蛋白、Per 3、锌指蛋白217、Krox-25和miwi 1。这些具有良好特征的基因和许多新基因为发育的早期阶段提供了标记，并为进一步的功能研究提供了起始材料。出版社：Elsevier爱尔兰Ltd.
As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies. Published by Elsevier Ireland Ltd.